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Synthesis of a hemoglobin-conjugated triblock copolymer for oxygen carrying and specific recognition of cancer cells

机译：血红蛋白共轭三嵌段共聚物的合成，用于携氧和癌细胞的特异性识别

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Tumour hypoxia, which has a negative effect on most anti-cancer treatments, has caused extensive concern over recent years. Concerned with this issue, this paper reports a hemoglobin-based oxygen nanocarrier (HBOC) functionalized with oxygen carrying and cancer cell recognition ability. First,?the triblock copolymer poly[2-(methacrylamido) glucopyranose]-b-poly(methacrylicacid)-b-poly(butylmethacrylate) (PMAG-b-PMAA-b-PBMA), in which the first block consists of glucose units, was synthesized and characterized. Following the self-assembly of the amphiphilic copolymer into micelles in phosphate buffer solution, hemoglobin was conjugated onto the micelles through a condensation reaction between the carboxyl groups on PMAA and the amino groups on Hb. The HBOC, with a mean diameter of around 147 nm, is stable and disperses homogeneously in phosphate buffer solution. In addition, an in vitro cytotoxicity study revealed that the HBOC is biocompatible. More importantly, this HBOC was demonstrated to hold excellent capacity for oxygen binding. Moreover, cellular uptake indicated that the HBOC was internalized more easily by cancer cells than normal cells, which means that the HBOC shows specific recognition of cancer cells. Therefore, the hemoglobin-conjugated polymer that we prepared could be a promising nanocarrier for tumour therapy.
机译：肿瘤低氧对大多数抗癌治疗均具有负面影响，近年来引起了广泛关注。关于这个问题，本文报道了一种基于血红蛋白的氧纳米载体（HBOC），其具有载氧和癌细胞识别功能。首先，三嵌段共聚物聚[2-（甲基丙烯酰胺基）吡喃葡萄糖]- b -聚（甲基丙烯酸）- b -聚（甲基丙烯酸丁酯）（PMAG- b （ -PMAA- b -PBMA），其中第一个嵌段由葡萄糖单元组成。将两亲共聚物在磷酸盐缓冲溶液中自组装成胶束后，血红蛋白通过PMAA上的羧基与Hb上的氨基之间的缩合反应缀合到胶束上。平均直径约为147 nm的HBOC是稳定的，并在磷酸盐缓冲溶液中均匀分散。此外，一项体外细胞毒性研究表明，HBOC具有生物相容性。更重要的是，这种HBOC被证明具有出色的氧结合能力。此外，细胞摄取表明HBOC比正常细胞更容易被癌细胞内化，这意味着HBOC显示出对癌细胞的特异性识别。因此，我们制备的结合血红蛋白的聚合物可能是用于肿瘤治疗的有前途的纳米载体。

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