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首页> 外文期刊>RSC Advances >Emission enhancement of GFP chromophore in aggregated state via combination of self-restricted effect and supramolecular host–guest complexation
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Emission enhancement of GFP chromophore in aggregated state via combination of self-restricted effect and supramolecular host–guest complexation

机译：通过自我限制效应和超分子宿主-客体复合作用的结合，增强聚集态中的GFP发色团的发射

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摘要

The emission enhancement of GFP-like chromophores in the aggregated state is a great challenge due to its free conformational motions and aggregation-induced quenching (ACQ) features. Herein, the emission response of a GFP chromophore (GFPc) in its aggregated state has been greatly enhanced via combination of self-restricted effect and supramolecular host–guest complexation. Specifically, the benzene group of the GFP chromophore (HBHI) was tailored by 2,5-methoxy group to construct a self-restricted chromophore (MBHI), which shows enhanced emission response in both solutions and aggregated state compared to HBHI due to the formation of the self-restricted effect. After conjugated to adamantine (AD), the chromophore (MBIAD) exhibits aggregation-induced emission enhancement (AIEE) feature compared to MBHI in aggregated state. Moreover, the aggregated emission of MBIAD further enhances by complexation with proper β-cyclodextrin (β-CD), which reduces strong ACQ between chromophores. Furthermore, the aggregated emission can also be tuned by controlling the ratio of β-CD and AD. Under a ratio of 1?:?2, MBIAD and Me-β-CD can assemble into nanoparticle with a diameter of about 55 nm, which has been applied to cell imaging due to the relatively low cell cytotoxicity.
机译：由于其自由的构象运动和聚集诱导的猝灭（ACQ）特征，聚集状态的GFP样发色团的发射增强是一个巨大的挑战。此处，通过自我限制效应和超分子宿主-客体复合作用的结合，大大增强了处于聚集状态的GFP发色团（GFPc）的发射响应。具体而言，GFP发色团（HBHI）的苯基由2,5-甲氧基修饰，以构建自限性发色团（MBHI），与HBHI相比，由于形成，在溶液和聚集状态下均显示出增强的发射响应自我限制的效果。与金刚烷（AD）偶联后，发色团（MBIAD）与聚集状态的MBHI相比，表现出聚集诱导的发射增强（AIEE）功能。此外，通过与适当的β-环糊精（β-CD）络合，MBIAD的聚集发射进一步增强，从而降低了生色团之间的强ACQ。另外，也可以通过控制β-CD与AD的比例来调整合计发射。 MBIAD和Me-β-CD的比例为1：2时，MBIAD和Me-β-CD可以组装成直径约55 nm的纳米颗粒，由于其相对较低的细胞毒性，已应用于细胞成像。

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