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首页> 外文期刊>RSC Advances >Control of ATP concentration in Escherichia coli using an ATP-sensing riboswitch for enhanced S-adenosylmethionine production
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Control of ATP concentration in Escherichia coli using an ATP-sensing riboswitch for enhanced S-adenosylmethionine production

机译：使用ATP感应核糖开关控制S.Adenosylmethionine产量的增加来控制大肠杆菌中的ATP浓度

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摘要

ATP is the limiting precursor for S-adenosylmethionine (SAM) synthesis in Escherichia coli. In contrast to traditional optimization of fermentation processes, the riboswitch-based strategy, which was developed as a novel efficient regulation approach, has been applied for the control of the intracellular ATP concentration in order to promote the production of SAM. In this work, a newly discovered ATP-sensing riboswitch ydaO motif was used to balance the requirement of ATP in SAM biosynthesis. The results show that the RNA element ydaO from Bacillus subtilis could play a role in regulating gene expression in E. coli. The ydaO motif-based regulation strategy, which was induced by ATP, improved the ATP level as well as the titer of SAM. The intracellular SAM titer of the best-performing strain, AYV, produced the highest SAM titer (1.23 mg L?1) which was increased by about 55% compared with the control. Overall, the method enabled new insights into the regulation of ATP in E. coli. These results demonstrate a strategy for optimally employing an ATP-sensing riboswitch for the ATP balance of industrial ATP-driven bioprocesses.
机译：ATP是大肠杆菌中合成S-腺苷甲硫氨酸（SAM）的限制性前体。与传统的发酵工艺优化相反，基于核糖开关的策略已被开发为一种新型的有效调节方法，已被用于控制细胞内ATP的浓度以促进SAM的产生。在这项工作中，新发现的ATP感应核糖开关 yda O基序被用来平衡SAM生物合成中ATP的需求。结果表明，枯草芽孢杆菌的RNA元件 yda O可能在调控 E基因表达中发挥作用。大肠杆菌。 ATP诱导的基于 yda O的调控策略提高了ATP水平以及SAM的效价。表现最佳的菌株AYV的细胞内SAM滴度产生最高的SAM滴度（1.23 mg L ？1 ），与对照组相比增加了约55％。 。总体而言，该方法使人们对 E中ATP的调控有了新的认识。大肠杆菌。这些结果证明了为工业ATP驱动的生物过程中的ATP平衡最佳地采用ATP传感核糖开关的策略。

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