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Tunable luminescence from two dimensional BCNO nanophosphor for high-contrast cellular imaging

机译：二维BCNO纳米磷光体的可调光用于高对比度细胞成像

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Rare-earth free and biocompatible two dimensional carbon based boron oxynitride (2D BCNO) nanophosphors were synthesized using facile auto-combustion of inexpensive compounds such as urea, boric acid and polyethylene glycol at ambient atmosphere and relatively low temperatures. The surface morphology and microstructure images indicate that the nanophosphor has 2D layered structures and analogous mixed hexagonal lattices of boron nitride (BN) and graphene (C). The nanophosphor exhibits a single, distinct and broad photoluminescence emission and this emission colour can be easily tuned from violet to deep red by varying the amount of boron/carbon content. The time-resolved and photoluminescence spectroscopic results indicate that B–O act as luminescence centers, which are responsible for the tunable luminescent properties while carbon impurities induce energy levels in the band gap of 2D BCNO nanophosphors. These tunable and biocompatible luminescent nanophosphors are used for in vitro high-contrast cellular imaging of HeLa cells derived from human cervical cancer cells as well as in vivo imaging in C57BL/6J mice. Hence, these novel multi-colour emitting nanophosphors provide a paradigm shift in rare-earth free biocompatible nanoprobes for next generation high-contrast in vitro and in vivo imaging applications.
机译：使用廉价的化合物（例如尿素，硼酸和聚乙二醇）在环境大气和相对较低的温度下轻松地自动燃烧，合成了无稀土且生物相容的二维碳基氮氧化硼纳米磷光体（2D BCNO）。表面形态和微观结构图像表明，纳米磷光体具有2D层状结构以及氮化硼（BN）和石墨烯（C）的类似混合六角形晶格。纳米磷光体表现出单一，独特和宽泛的光致发光发射，并且通过改变硼/碳含量，可以容易地将发射光的颜色从紫色调至深红色。时间分辨和光致发光光谱结果表明，BO充当发光中心，负责可调节的发光特性，而碳杂质在二维BCNO纳米磷的带隙中诱导能级。这些可调的且生物相容的发光纳米荧光粉用于人类宫颈癌细胞HeLa细胞的体外高对比度细胞成像以及C57BL / 6J小鼠的体内成像。因此，这些新颖的发射多种颜色的纳米磷光体为下一代高对比度体外和体内成像应用提供了无稀土生物相容性纳米探针的范例转变。

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