Targeted isolation of sulfur-containing metabolites from <em>Lsr2</em>-deletion mutant strain of <em>Streptomyces roseosporus</em>

Lina Deng; Rui Wang; Guowei Wang; Mingxu Liu; Guojian Liao; Zhihua Liao; Min Chen

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Targeted isolation of sulfur-containing metabolites from Lsr2-deletion mutant strain of Streptomyces roseosporus

机译：迷迭香链霉菌的 Lsr2 缺失突变菌株的目标硫分离代谢

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Deletion of the Lsr2 gene in Streptomyces roseosporus up-regulated silent gene clusters and produced new secondary metabolites. An ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF-MS/MS) method was used to analyze metabolites of the mutant and wild-type strains, and recognize previously unreported sulfur-containing compounds based on their molecular formulas and fragmentation ions. The targeted isolation of unidentified compounds afforded six new sulfur-containing compounds, pyrismycins A–F (1–6), together with seven known analogues 7–13. Their cytotoxic effects were evaluated using four clinically relevant human cancer cell lines, gastric carcinoma SGC7901, breast carcinoma MDA-MB-231, lung carcinoma A549 and hepatocellular carcinoma HepG2. Compound 7 exhibited the most potent cytotoxicity with IC₅₀ values of 1.7, 5.8 and 6.3 μM against the SGC7901, HepG2 and MDA-MB-231, respectively. 展开▼

机译：玫瑰链霉菌中 Lsr2 基因的缺失上调沉默基因簇并产生新的次生代谢产物。使用超高效液相色谱四极杆飞行时间质谱（UPLC-QTOF-MS / MS）方法分析突变菌株和野生型菌株的代谢产物，并根据其分子式和分子式识别先前未报告的含硫化合物。碎片离子。通过对未鉴定化合物的靶向分离，得到了六种新的含硫化合物，嘧菌霉素A-F（1-6），以及七种已知的类似物7-13。使用四种临床相关的人类癌细胞系胃癌SGC7901，乳腺癌MDA-MB-231，肺癌A549和肝细胞癌HepG2评估了它们的细胞毒性作用。化合物7对SGC7901，HepG2和MDA-MB-231表现出最强的细胞毒性，IC _{50 值分别为1.7、5.8和6.3μM。} 展开▼

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《RSC Advances》 |2017年第60期|共7页

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Lina Deng; Rui Wang; Guowei Wang; Mingxu Liu; Guojian Liao; Zhihua Liao; Min Chen;
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中图分类化学;

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专利

1. Strain Improvement of Streptomyces roseosporus for Daptomycin Production by Rational Screening of He–Ne Laser and NTG Induced Mutants and Kinetic Modeling [J] . Guanghai Yu, Xiaoqiang Jia, Jianping Wen, Applied Biochemistry and Biotechnology . 2011,第6期

机译：通过合理筛选He-Ne激光和NTG诱导的突变体并建立动力学模型来改进玫瑰孢链霉菌生产达托霉素的菌株

2. Strain improvement of Streptomyces roseosporus for daptomycin production by rational screening of He-Ne laser and NTG induced mutants and kinetic modeling [J] . Yu G., Jia X., Wen J., Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology . 2011,第6期

机译：通过合理筛选He-Ne激光和NTG诱导的突变体和动力学建模来改善玫瑰孢链霉菌用于达托霉素生产的菌株

3. Isolation and Characterization of New 16-Membered Macrolides from the aveA3 Gene Replacement Mutant Strain Streptomyces avermitilis TM24 with Acaricidal and Nematicidal Activities [J] . Feng Youling, Yu Zhen, Zhang Shaoyong, Journal of Agricultural and Food Chemistry . 2019,第17期

机译：来自ARGEA3基因替代突变体菌株中霉菌菌株HEREMITILIS TM24的新的16元大胶质剂的分离与表征

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机译：通过在蓝色细菌中的系统基因破坏系统中，突变中的高CO_2分离，SneCechocystis Sp。 PCC6803

5. ISOLATION OF MOLONEY LEUKEMIA VIRUS TEMPERATURE SENSITIVE MUTANTS, AND THE CHARACTERIZATION OF A TEMPERATURE SENSITIVE MUTANT DEFICIENT IN THE INCORPORATION OF ENV GENE PROTEINS [D] . GALLICK, GARY EDWARD. 1981

机译：包膜ENV基因蛋白对人白血球病毒温度敏感性突变体的分离及温度敏感性突变体的表征

6. Development of a mechanism of action-based screen for anthelmintic microbial metabolites with avermectinlike activity and isolation of milbemycin-producing Streptomyces strains. [O] . C L Haber, C L Heckaman, G P Li, 1991

机译：基于行动的筛选机制的蠕虫微生物代谢产物具有阿维菌素样活性并分离出产米尔贝霉素的链霉菌菌株。

7. Isolation of viable deletion mutants of temperate phage SAt1 in Streptomyces azureus and a restriction analysis of their DNAs. [O] . HIKARU SUENAGA, SHINSAKU HAYASHIDA, SEIYA OGATA 1989

机译：温带噬菌体SAT1中活缺失突变体的分离及其DNA的限制分析。

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机译：野生型，突变体和重组'链霉菌'在土壤生态系统中的存活和影响

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3. PHASEMATION EA-1000模拟相关采用左右独立结构、分离电源箱体,以“终极模拟回放”为目标的唱机EQ [J] . 小林贡 . 现代音响技术 . 2013,第002期

4. HORIBA公司EMIA-20系列高频红外碳硫仪故障分析与排除 [C] . 罗文 . 2007年国产科学仪器应用、创新和产业化学术研讨会 . 2007

1. meridamycin synthase complex, host cell, synthetic polyketide synthase, nucleic acid sequence, recombinant vector, isolated nucleic acid, chimeric nucleic acid construction, methods for producing a polyketide compound and for determining the structural requirement for polyketide activity, meridamycin, mutant streptomyces strain, keto meridamycin c36 or a pharmaceutically acceptable salt thereof, deoximeridamycin compound, pharmaceutically acceptable salts or mixtures thereof, pharmaceutical composition, use of a compound, actinomycete strain m507, and methods for producing a macrolide and to produce a compound [P] . 外国专利： BRPI0511777A . 2008-01-15

机译：梅里达霉素合酶复合物，宿主细胞，合成的聚酮化合物合酶，核酸序列，重组载体，分离的核酸，嵌合核酸的构建，制备聚酮化合物的方法和确定聚酮化合物活性的结构要求的方法，美利霉素，突变链霉菌菌株，酮meridamycin c36或其药学上可接受的盐，脱氧米里达霉素化合物，药学上可接受的盐或混合物，药物组合物，该化合物的用途，放线菌菌株m507以及制备大环内酯和制备化合物的方法

2. Fermentation process for preparing 7-chloro-6-demethyltetracycline (I) using mutant strains of Streptomyces aureofaciens which impart a colour to their whole ha [P] . 外国专利： CH501727A . 1971-01-15

机译：利用金黄色链霉菌突变株制备其7-氯-6-去甲基四环素（I）的发酵方法

3. Isolation method of the new high-efficiency mutant strain of plants and fungi due to enhancement of the molecules involved in the process and the elucidation of the erasing process of singlet oxygen and / or [P] . 外国专利： JPWO2009096443A1 . 2011-05-26

机译：由于增强了参与该过程的分子并阐明了单线态氧和/或清除过程的分子，因此新的植物和真菌高效突变株的分离方法

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Targeted isolation of sulfur-containing metabolites from Lsr2-deletion mutant strain of Streptomyces roseosporus

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