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Adsorption mechanism of mussel-derived adhesive proteins onto various self-assembled monolayers

机译：贻贝来源的粘附蛋白在各种自组装单分子层上的吸附机理

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Mussel adhesion to a variety of surfaces has received considerable attention due to its ability to bind strongly to many surfaces under water. Understanding the interactions between mussel-derived adhesive proteins and surfaces with different chemical and physical properties is of great theoretical and practical interest. Here, we explored the adsorption behavior of mussel foot protein-1 (Mfp-1) onto self-assembled monolayers (SAMs) with varying wettability and chemistry, through quartz crystal microbalance with dissipation measurements, ellipsometry, X-ray photoelectron spectroscopy, and atomic force microscopy. The results showed significant differences in the structural conformations of protein adsorbed layers for the series of surfaces. Two mechanisms were found in all the systems; in the case of hydrophobic surfaces, the first regime corresponded to the initial adsorption of protein molecules onto the surfaces, and the second kinetic process was related to conformational changes, resulting in a relatively rigid and dense protein layer; while for hydrophilic surfaces, a loose and soft adsorbed protein film was generated. It was found that the adsorbed mass of Mfp-1 at the hydroxyl terminated SAM surface was the smallest among all the modified surfaces, because of the formation of hydration layers reducing protein adhesion effectively. Furthermore, the interaction mechanisms of protein molecules with solid surfaces were suggested, providing a new way of designing and developing underwater adhesive or anti-fouling materials.
机译：贻贝对各种表面的粘附力因其在水下牢固结合许多表面的能力而备受关注。了解贻贝来源的粘附蛋白与具有不同化学和物理性质的表面之间的相互作用具有重大的理论和实践意义。在这里，我们通过具有耗散测量，椭圆偏振法，X射线光电子能谱和原子能谱的石英晶体微量天平，探索了贻贝足蛋白1（Mfp-1）在具有不同润湿性和化学性质的自组装单分子膜（SAMs）上的吸附行为。力显微镜。结果表明，在一系列表面上，蛋白质吸附层的结构构象存在显着差异。在所有系统中发现了两种机制。在疏水性表面的情况下，第一个过程对应于蛋白质分子在表面上的初始吸附，第二个动力学过程与构象变化有关，从而导致相对刚性和致密的蛋白质层。而对于亲水性表面，会产生疏松而柔软的蛋白膜。发现在羟基修饰的SAM表面上Mfp-1的吸附质量在所有改性表面中最小，这是因为形成了水合层，有效地降低了蛋白质的粘附。此外，提出了蛋白质分子与固体表面的相互作用机理，为设计和开发水下胶粘剂或防污材料提供了一种新方法。

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