Enhanced fuel ethanol production from rice straw hydrolysate by an inhibitor-tolerant mutant strain of <em>Scheffersomyces stipitis</em>

Kedong Ma; Mingxiong He; Huiyan You; Liwei Pan; Guoquan Hu; Yubo Cui; Toshinari Maeda

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Enhanced fuel ethanol production from rice straw hydrolysate by an inhibitor-tolerant mutant strain of Scheffersomyces stipitis

机译：耐受性强弱毒杆菌突变株提高稻草水解物的燃料乙醇产量

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The aim of the present study was to develop an inhibitor-tolerant strain of Scheffersomyces stipitis and establish an efficient ethanol fermentation process for cost-effective ethanol production from lignocellulosic biomass. By a strategy of three successive rounds of UV mutagenesis following adaptation, we isolated a S. stipitis mutant with improved tolerance against ethanol and inhibitors in the form of acetic acid, furfural and vanillin. The mutant strain exhibited excellent ethanol fermentation performance; both the xylose and glucose consumption rate and ethanol productivity were almost two times higher than the parental strain in batch fermentation. To overcome the issue of product inhibition and carbon catabolite repression (CCR) effect, the membrane integrated continuous fermentation system was employed. The maximum ethanol titer of 43.2?g l?1 and productivity of 2.16 g l?1 h?1 was achieved at a dilution rate of 0.05 h?1, higher than the relevant studies ever reported. These results suggested the novel process of cell recycling continuous fermentation using S. stipitis mutant has great potential for commercial ethanol production from lignocelluloses-based biomass.

机译：本研究的目的是开发一种耐受性强的拟南芥（Scheffersomyces stipitis）的菌株，并建立一种有效的乙醇发酵工艺，以从木质纤维素生物质中经济高效地生产乙醇。通过适应后连续三轮紫外线诱变的策略，我们分离出了 S。改良的对乙醇的耐受性突变体，包括乙酸，糠醛和香兰素。该突变菌株表现出优异的乙醇发酵性能。在分批发酵中，木糖和葡萄糖消耗率以及乙醇生产率几乎都是亲本菌株的两倍。为了克服产物抑制和碳分解代谢物抑制（CCR）效应的问题，采用了膜集成连续发酵系统。最大乙醇滴度为43.2？gl ？1 ，生产率为2.16 gl ？1 h < sup>？1 的稀释度为0.05 h ？1 ，高于相关报道。这些结果表明使用 S进行细胞再循环连续发酵的新过程。 stipitis 突变体具有从木质纤维素基生物质生产乙醇的巨大潜力。 展开▼

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《RSC Advances》 |2017年第50期|共9页

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Kedong Ma; Mingxiong He; Huiyan You; Liwei Pan; Guoquan Hu; Yubo Cui; Toshinari Maeda;
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1. Enhanced ethanol production by fermentation of rice straw hydrolysate without detoxification using a newly adapted strain of Pichia stipitis [J] . Huang CF, Lin TH, Guo GL, Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies . 2009,第17期

机译：通过使用新适应的毕赤酵母菌株对稻草水解产物进行发酵而无需解毒，从而提高了乙醇的产量

2. Detoxification of Rice Straw and Olive Tree Pruning Hemicellulosic Hydrolysates Employing Saccharomyces cerevisiae and Its Effect on the Ethanol Production by Pichia stipitis [J] . Bruno Guedes Fonseca, Juan Gabriel Puentes, Soledad Mateo Journal of Agricultural and Food Chemistry . 2013,第40期

机译：用酿酒酵母对稻草和橄榄树修剪半纤维素水解产物的解毒及其对毕赤酵母的乙醇生产的影响

3. Assessment of advanced oxidative processes based on heterogeneous catalysis as a detoxification method of rice straw hemicellulose hydrolysate and their effect on ethanol production by Pichia stipitis [J] . Jo?o Paulo Alves Silva, Livia Melo Carneiro, Inês Concei??o Roberto Biomass Conversion and Biorefinery . 2014,第3期

机译：基于多相催化作为稻草半纤维素水解产物解毒方法的高级氧化过程及其对树干毕赤酵母产乙醇的影响

4. Ethanol production from rice straw hydrolysates by Pichia stipitis [C] . Wen-Heng Chen, Ting-Shiang Lin, Gia-Luen Guo International Conference on Advances in Energy Engineering . 2013

机译：荔枝秸秆水解素的乙醇产量由毕赤酵母

5. Strain selection and metabolic engineering of Pichia stipitis for increased ethanol production from xylose. [D] . Dahn, Kristine Mary. 1998

机译：毕赤氏毕赤酵母的菌株选择和代谢工程，以提高木糖生产乙醇的能力。

6. Enhanced bioethanol production from wheat straw hemicellulose by mutant strains of pentose fermenting organisms Pichia stipitis and Candida shehatae [O] . Sravanthi Koti, Sai Prashanthi Govumoni, Jahnavi Gentela, -1

机译：戊糖发酵生物毕赤酵母和假丝酵母念珠菌突变株提高了小麦秸秆半纤维素的生物乙醇生产

7. Enhanced fuel ethanol production from rice straw hydrolysate by an inhibitor-tolerant mutant strain of Scheffersomyces stipitis [O] . Ma Kedong, He Mingxiong, You Huiyan, 2017

机译：耐受性强毒裂殖酵母突变株从稻草水解物中提高燃料乙醇的生产

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5. 异源过表达OsATG8b基因提高转基因拟南芥的氮/碳胁迫耐受性和产量 [J] . 甄晓溪 ,刘浩然 ,李鑫 . 植物学报 . 2019,第001期

6. 乳酸脱氢酶2基因的缺失提高了苏云金芽胞杆菌的碱耐受性 [C] . 考桂伟 ,邱丽丽 ,彭琦 . 全国农业生物化学与分子生物学第十四届学术研讨会 . 2015

7. Mn、Fe(Ⅱ)元素提高稻草秸秆制备燃料乙醇产率的研究 [A] . 毕智明 . 2013

1. 一种提高稻草制燃料乙醇产率的新方法 [P] . 中国专利： CN103074407A . 2013-05-01

2. 一株鸭疫里默氏杆菌的减毒突变株及其应用 [P] . 中国专利： CN107964526A . 2018-04-27

3. Scheffersomyces stipitis strain for increased ethanol production and uses thereof [P] . 外国专利： US8609382B1 . 2013-12-17

机译：用于提高乙醇产量的裂殖酵母菌株及其用途

4. '' method for producing a saccharomyces cerevisiae strain with introduced genes encoding xylose reductase, xylitol dehydrogenase and xylulokinase and with enhanced ethanol production, enhanced xylose conversion and reduced axitol production, introduced saccharomyces cerevisiae strain encoding xylose reductase , with enhanced ethanol production, xylitol dehydrogenase and xylulokinase, enhanced xylose conversion and reduced xylitol production, enhanced ethanol producing saccharomyces cerevisiae strain, enhanced xylose conversion and reduced xylitol production, ethanol producing saccharomyces cerevisiae strain enhanced xylose conversion, reduced xylitol production, enhanced ethanol producing saccaromyces cerevisiae strain, enhanced xylose conversion, enhanced ethanol production, reduced xylitol production, and increased inhibitor tolerance and use of a '' strain [P] . 外国专利： BR112013011815A2 . 2017-10-31

机译：''生产具有引入的编码木糖还原酶，木糖醇脱氢酶和木酮糖酶基因的酿酒酵母菌株的方法，并具有增强的乙醇产量，增强的木糖转化率和减少的木糖醇产量，引入引入的编码木糖还原酶的酿酒酵母菌株，增强的乙醇产量，木糖醇脱氢酶和木酮糖激酶，提高的木糖转化率和降低的木糖醇产量，提高的乙醇生产酵母菌菌株，提高的木糖转化率和减少的木糖醇产量，乙醇生产的酿酒酵母菌株，增强的木糖转化率，降低的木糖醇产量，提高的乙醇生产酵母菌，提高的木糖酵母转化率，提高的木糖转化率，乙醇生产，木糖醇生产减少，抑制剂耐受性提高和使用''菌株

5. ETHANOL PRODUCTION PROCESS BY CONTINUOUS FERMENTATION FROM YEAST CELLS (SCHEFFERSOMYCES STIPITIS) IMMOBILIZED IN PRETREATED CANE BAGASSE. [P] . 外国专利： MX2015005129A . 2016-10-21

机译：通过在预制的甘蔗袋中固定化的酵母细胞（拟南芥）的连续发酵生产乙醇。

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Enhanced fuel ethanol production from rice straw hydrolysate by an inhibitor-tolerant mutant strain of Scheffersomyces stipitis

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