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首页> 外文期刊>RSC Advances >Studies on effect of Ginkgo biloba?L. leaves in acute gout with hyperuricemia model rats by using UPLC-ESI-Q-TOF/MS metabolomic approach
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Studies on effect of Ginkgo biloba?L. leaves in acute gout with hyperuricemia model rats by using UPLC-ESI-Q-TOF/MS metabolomic approach

机译：银杏叶的功效研究。 UPLC-ESI-Q-TOF / MS代谢组学方法研究高尿酸血症模型大鼠急性痛风

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Gout is a result of sodium urate deposition in and around the joints that is caused by long-standing hyperuricemia. In the present study, Ginkgo biloba L. leaves have been used for the treatment of acute gout and hyperuricemia, but the pathogenesis of acute gout with hyperuricemia and the mechanism of action of Ginkgo biloba L. leaves in gout remain unclear. This study aimed to investigate the pathogenesis of acute gout with hyperuricemia in rats and investigate the therapeutic effects of extract of Ginkgo biloba L. leaves (GBE) by using the metabolomic method. In this study, the rat model of acute gout with hyperuricemia was established by intraperitoneal injection of xanthine and oxonic acid potassium salt and intra-articular injection of monosodium urate (MSU). Serum level of interleukin-1 beta (IL-1β) was evaluated to compare the model group with the group with GBE by enzyme-linked immunosorbent assay (ELISA). Joint swelling was used for testing the effects of MSU and the pathological changes of joint and kidney were assessed by hematoxylin–eosin (H&E) staining. Potential biomarkers were identified from urinary samples by ultra-performance liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS) combined with principal component analysis (PCA) which was used to observe the metabonomic alterations and the separations of the scatter points of different groups. Results show that joint swelling and serum level of IL-1β significantly decreased, and pathological abnormalities of the joint and kidney were ameliorated after GBE treatment. 27 potential biomarkers were identified and the primary metabolism pathways involved tryptophan metabolism, pyrimidine metabolism, pentose phosphate pathway, TCA cycle, tyrosine metabolism, lysine degradation and purine metabolism. The disturbed pathways were restored after treatment with GBE. This study indicated that GBE possessed evident therapeutic effects on acute gout with hyperuricemia rats.
机译：痛风是由于长期高尿酸血症引起尿酸钠在关节内和关节周围沉积的结果。在本研究中，银杏叶已被用于治疗急性痛风和高尿酸血症，但是急性痛风伴高尿酸血症的发病机理和银杏叶的作用机理 em>痛风中的落叶仍不清楚。本研究旨在探讨大鼠高尿酸血症急性痛风的发病机制，并通过代谢组学方法研究银杏叶提取物（GBE）的治疗作用。在这项研究中，通过腹膜内注射黄嘌呤和氧酸钾盐并关节内注射尿酸一钠（MSU）建立急性高尿酸血症痛风模型。通过酶联免疫吸附测定（ELISA），评估血清白介素-1β（IL-1β）水平以将模型组与具有GBE的组进行比较。关节肿胀用于测试MSU的作用，并通过苏木精-伊红（H＆E）染色评估关节和肾脏的病理变化。通过超高效液相色谱-电喷雾电离四极杆飞行时间质谱（UPLC-ESI-Q-TOF / MS）和用于观察代谢组学的主成分分析（PCA），从尿液样品中鉴定出了潜在的生物标志物不同组的散射点的变化和分离。结果表明GBE治疗后关节肿胀和血清IL-1β水平明显降低，关节和肾脏的病理异常得到改善。确定了27种潜在的生物标志物，主要的代谢途径涉及色氨酸代谢，嘧啶代谢，磷酸戊糖途径，TCA循环，酪氨酸代谢，赖氨酸降解和嘌呤代谢。 GBE治疗后恢复了受干扰的通路。该研究表明GBE对高尿酸血症大鼠急性痛风具有明显的治疗作用。

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