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首页> 外文期刊>RSC Advances >A highly sensitive electrochemical impedance immunosensor for indole-3-acetic acid and its determination in sunflowers under salt stress
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A highly sensitive electrochemical impedance immunosensor for indole-3-acetic acid and its determination in sunflowers under salt stress

机译：吲哚-3-乙酸的高灵敏度电化学阻抗免疫传感器及其在盐胁迫下向日葵中的测定

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摘要

As an important phytohormone, indole-3-acetic acid (IAA) plays an essential role during the whole process of growth and development of plants. In this work, a novel label-free electrochemical impedance immunosensor for IAA determination has been developed. A three-dimensional PAMAM dendrimer was applied to improve greatly the immobilization capacity of antibodies for IAA on a gold electrode. Gold nanoparticles (AuNPs) were used to further improve the sensitivity of the biosensor by forming complexes with anti-IAA antibodies. The developed impedance immunosensor shows ultra-sensitivity, high selectivity and good reliability for IAA detection. The IAA extracted from the stems of sunflowers under different salt stress conditions was successfully detected using the immunosensor. The results are in good agreement with those obtained by UPLC-MS and consistent with published data, thus confirming the practical value of the biosensor. To the best of our knowledge, this is the first electrochemical impedance immunosensor for IAA detection, which provides a promising detection platform for IAA. By replacing antibodies, this strategy can be easily used to detect other phytohormones.
机译：吲哚-3-乙酸（IAA）作为重要的植物激素，在植物生长发育的整个过程中都起着至关重要的作用。在这项工作中，已开发出用于IAA测定的新型无标记电化学阻抗免疫传感器。应用三维PAMAM树枝状聚合物可大大提高IAA抗体在金电极上的固定能力。金纳米颗粒（AuNPs）用于通过与抗IAA抗体形成复合物来进一步提高生物传感器的灵敏度。研发的阻抗免疫传感器对IAA检测具有超高灵敏度，高选择性和良好的可靠性。使用免疫传感器成功检测了在不同盐胁迫条件下从向日葵茎中提取的IAA。结果与通过UPLC-MS获得的结果非常一致，并且与公开的数据一致，从而证实了该生物传感器的实用价值。据我们所知，这是第一款用于IAA检测的电化学阻抗免疫传感器，为IAA提供了有希望的检测平台。通过替换抗体，该策略可轻松用于检测其他植物激素。

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