Anaerobic lipopeptide biosurfactant production by an engineered bacterial strain for <em>in situ</em> microbial enhanced oil recovery

Xiaolong Liang; Rongjiu Shi; Mark Radosevich; Feng Zhao; Yingyue Zhang; Siqin Han; Ying Zhang

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Anaerobic lipopeptide biosurfactant production by an engineered bacterial strain for in situ microbial enhanced oil recovery

机译：工程菌菌株生产原位微生物的厌氧脂肽生物表面活性剂可提高采油率

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Bacillus mojavensis JF-2 produces water-soluble lipopeptide under aerobic conditions, while Pseudomonas stutzeri DQ1 grows rapidly under anaerobic conditions. These bacteria were used to construct an engineered strain for anaerobic lipopeptide production by protoplast fusion for potential use in microbially enhanced oil recovery (MEOR). The resulting fusant strain FA-2 produced lipopeptide (382 mg l?1) anaerobically at temperatures up to 50 °C, across a pH range of 4.5–10.0, and at salt concentrations as high as 10% NaCl. Experimental results from a physical simulation core at 39 °C suggest that FA-2 has potential for use in MEOR. 展开▼

机译：莫哈韦芽孢杆菌 JF-2在好氧条件下产生水溶性脂肽，而施氏假单胞菌 DQ1在厌氧条件下快速生长。这些细菌用于构建原生质体融合生产厌氧脂肽的工程菌株，可用于微生物强化采油（MEOR）。最终的融合菌株FA-2在高达50°C的温度，4.5–10.0的pH范围和温度下，厌氧产生脂肽（382 mg l ？1 ）。盐浓度高达10％NaCl。物理模拟核心在39°C下的实验结果表明，FA-2具有用于MEOR的潜力。 展开▼

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《RSC Advances》 |2017年第33期|共10页

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Xiaolong Liang; Rongjiu Shi; Mark Radosevich; Feng Zhao; Yingyue Zhang; Siqin Han; Ying Zhang;
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中图分类化学;

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1. Anaerobic lipopeptide biosurfactant production by an engineered bacterial strain for in situ microbial enhanced oil recovery [J] . Xiaolong Liang, Rongjiu Shi, Mark Radosevich, RSC Advances . 2017,第33期

机译：工程菌菌株生产原位微生物的厌氧脂肽生物表面活性剂可提高采油率

2. Modification of Interfacial Tension and Wettability in Oil-Brine- Quartz System by in Situ Bacterial Biosurfactant Production at Reservoir Conditions: Implications for Microbial Enhanced Oil Recovery [J] . Park Taehyung, Jeon Min-Kyung, Yoon Sukhwan, Energy & fuels . 2019,第JUNa期

机译：油藏条件下原位细菌生物表面活性剂生产对油-盐-石英系统界面张力和润湿性的改变：对微生物强化采油的意义

3. In-situ lipopeptide biosurfactant production by Bacillus strains correlates with improved oil recovery in two oil wells approaching their economic limit of production [J] . Youssef Noha, Simpson D. Randall, McInerney Michael J., International Biodeterioration & Biodegradation . 2013,第Null期

机译：芽孢杆菌属菌株的原位脂肽生物表面活性剂生产与两个油井中接近其生产极限的油井采收率提高有关

4. INJECTION OF RHAMNOLIPID (BIOSURFACTANT) FROM GENETICALLY ENGINEERED THERMUS AQUATICUS AND CHEMICAL SURFACTANT FOLLOWING HOT WATER INJECTION TO ENHANCE OIL RECOVERY [C] . Faustina Prima Martha Annual convention of the indonesian petroleum association . 2018

机译：在热水注射后从遗传工程热药物和化学表面活性剂注射rhamnolipid（生物活性剂），以提高采油

5. Microbial biosurfactant production: The effect of the Bacillus strain JF-2 biosurfactant on anaerobic hydrocarbon degradation, and the presence of indigenous biosurfactant-producing bacteria in soils. [D] . Jennings, Eleanor Mary. 2005

机译：微生物生物表面活性剂的生产：芽孢杆菌JF-2生物表面活性剂对厌氧性烃降解的影响以及土壤中存在产生生物表面活性剂的细菌。

6. Production of lipopeptide biosurfactants by Bacillus atrophaeus 5-2a and their potential use in microbial enhanced oil recovery [O] . Junhui Zhang, Quanhong Xue, Hui Gao, 2016

机译：萎缩芽孢杆菌5-2a产生脂肽生物表面活性剂及其在微生物强化采油中的潜在用途

7. Modification of Interfacial Tension and Wettability in Oil–Brine–Quartz System by in Situ Bacterial Biosurfactant Production at Reservoir Conditions: Implications for Microbial Enhanced Oil Recovery [O] . Taehyung Park, Min-Kyung Jeon, Sukhwan Yoon, 2019

机译：储层条件下原位细菌生物活性剂生产的界面张力和润湿性的改变：微生物增强的储存的影响

8. Development of Improved Anaerobic Growth of 'Bacillus Mojavensis' Strain JF-2 for the purpose of Improved Anaerobic Biosurfactant Production for Enhanced Oil Recovery. Topical Report, June 1, 2002-May 31, 2004 [R] . McInerney, M. J., Folmsbee, M. 2004

机译：开发改良的'芽孢杆菌'菌株JF-2厌氧生长，以改善厌氧生物表面活性剂生产，提高采收率。专题报告，2002年6月1日至2004年5月31日

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1. 一种大肠杆菌工程菌株及其好氧-微好氧-厌氧全阶段发酵生产琥珀酸的应用 [P] . 中国专利： CN103243064B . 2015.04.22

2. 一种大肠杆菌工程菌株及其好氧-微好氧-厌氧全阶段发酵生产琥珀酸的应用 [P] . 中国专利： CN103243064A . 2013-08-14

3. A steady state anaerobic denitrifying consortium for application in in-situ bioremediation of hydrocarbon-contaminated sites and enhanced oil recovery [P] . 外国专利： GB2511673B . 2014-12-10

机译：稳态厌氧反硝化联合体，用于烃污染位点的原位生物修复和提高采油率

4. A steady state anaerobic denitrifying consortium for application in in-situ bioremediation of hydrocarbon-contaminated sites and enhanced oil recovery [P] . 外国专利： GB2511673A . 2014-09-10

机译：稳态厌氧反硝化联合体，用于烃污染位点的原位生物修复和提高采油率

5. A steady state anaerobic denitrifying consortium for application in in-situ bioremediation of hydrocarbon-contaminated sites and enhanced oil recovery [P] . 外国专利： GB2483170B . 2014-10-22

机译：稳态厌氧反硝化联合体，用于烃污染位点的原位生物修复和提高采油率

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Anaerobic lipopeptide biosurfactant production by an engineered bacterial strain for in situ microbial enhanced oil recovery

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