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首页> 外文期刊>RSC Advances >Determination of berberine hydrochloride using a fluorimetric method with silica nanoparticles as a probe
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Determination of berberine hydrochloride using a fluorimetric method with silica nanoparticles as a probe

机译：以二氧化硅纳米粒子为探针的荧光法测定盐酸小ber碱

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The interaction of silica nanoparticles (SiO _(2) NPs) with berberine hydrochloride (BRH) was studied in aqueous solution at pH 9.0 and room temperature by using fluorophotometry. Based on a significant enhancement of the fluorescence intensity of the SiO _(2) NPs–BRH aggregates, a spectrofluorimetric method which was simple, sensitive and green was developed for the determination of BRH in aqueous solution. The linear range of the method was from 2.0–50.0 μg L ~(?1) with a detection limit of 0.73 μg L ~(?1) . There was no interference from the compounds normally used to formulate pharmaceutical tablets. The proposed method was applied to the determination of BRH in tablets with satisfactory results and good consistency with the results obtained by standard methods.
机译：利用荧光分光光度法研究了二氧化硅纳米颗粒（SiO_（2）NPs）与盐酸小ber碱（BRH）的相互作用。基于SiO _（2）NPs-BRH聚集体的荧光强度的显着增强，开发了一种简单，灵敏，绿色的分光荧光法测定水溶液中的BRH。该方法的线性范围为2.0-50.0μgL〜（？1），检测极限为0.73μgL〜（？1）。通常用于配制药物片剂的化合物没有干扰。该方法用于片剂中BRH的测定，结果令人满意，与标准方法的结果吻合良好。

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