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首页> 外文期刊>RSC Advances >Preparation, characterization and reusability efficacy of amine-functionalized graphene oxide-polyphenol oxidase complex for removal of phenol from aqueous phase
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Preparation, characterization and reusability efficacy of amine-functionalized graphene oxide-polyphenol oxidase complex for removal of phenol from aqueous phase

机译：胺官能化氧化石墨烯-多酚氧化酶复合物从水相中去除苯酚的制备，表征及重用性

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The present study explores the preparation, characterization and reusability efficacy of an amine-functionalized graphene oxide and polyphenol oxidase complex for the removal of phenol from aqueous phase. In brief, graphene oxide (GO) is synthesized according to modified Hummer's method using graphite powder and functionalized with amine using the Bucherer's method (GO-NH _(2) ). Partially purified polyphenol oxidase (PP-PPO) enzyme extracted from Solanum tuberosum is used for the preparation of the complex. The resultant GO-NH _(2) -(PP-PPO) complex is used for the phenol degradation studies. The samples of GO, GO-NH _(2) , and GO-NH _(2) -(PP-PPO) complex are characterized using various instrumental techniques. Spectral UV data and FTIR and XRD diffraction patterns confirm the amine functionalization on GO. Raman spectrum, SEM micrograph and thermogravimetric (TG) analyses authenticate the linked enzyme on GO-NH _(2) . GO-NH _(2) -(PP-PPO) complex demonstrates >90% enzyme stability at all the studied temperatures (4 °C, ?20 °C, RT and 37 °C). Phenol degradation studies show >99% removal of 1000 ppm of phenol within 5 hours from the start of the experiment at the optimized pH of 5.0 and temperature of 30 °C, as inferred from HPLC analysis. Catechol and hydroquinone compounds are identified as intermediates during the removal of phenol. Furthermore, studies on the reuse of GO-NH _(2) -(PP-PPO) complex suggest that the complex can be used effectively for the removal of phenol up to maximum 7 cycles. In conclusion, the observations made in the present study show that the complex containing amine-functionalized graphene oxide and phenoloxidase is effective for the removal of phenol with appreciable reusability.
机译：本研究探索了一种胺官能化的氧化石墨烯和多酚氧化酶复合物的制备，表征和可重复使用的功效，该复合物可从水相中去除苯酚。简而言之，氧化石墨烯（GO）是根据改良的悍马方法（使用石墨粉）合成的，并使用布彻勒方法（GO-NH _（2））用胺官能化。从马铃薯中提取的部分纯化的多酚氧化酶（PP-PPO）酶用于制备复合物。所得的GO-NH_（2）-（PP-PPO）配合物用于苯酚降解研究。使用各种仪器技术对GO，GO-NH_（2）和GO-NH_（2）-（PP-PPO）配合物的样品进行表征。光谱UV数据以及FTIR和XRD衍射图证实了GO上的胺官能化。拉曼光谱，SEM显微照片和热重分析（TG）分析验证了GO-NH_（2）上的连接酶。 GO-NH_（2-）-（PP-PPO）配合物在所有研究温度（4°C，？20°C，RT和37°C）下均显示> 90％的酶稳定性。苯酚降解研究表明，从最佳实验开始，在5个小时的最佳pH值为5.0和温度为30°C的情况下，从实验开始的5个小时内，去除了1000 ppm的苯酚> 99％，如HPLC分析所示。苯酚和对苯二酚化合物被确定为苯酚去除过程中的中间体。此外，对GO-NH_（2）-（PP-PPO）配合物的再利用的研究表明，该配合物可有效用于苯酚的去除，最多可进行7个循环。总之，本研究的观察结果表明，含有胺官能化氧化石墨烯和酚氧化酶的配合物可有效去除苯酚，并具有相当的可重复使用性。

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