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首页> 外文期刊>RSC Advances >A rapid immunochromatographic strip for neutralizing antibodies detection of foot and mouth disease virus serotype O
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A rapid immunochromatographic strip for neutralizing antibodies detection of foot and mouth disease virus serotype O

机译：快速中和抗体检测O型口蹄疫病毒的快速免疫层析带

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Four peptides were designed and synthesized according to the main FMDV type O vaccine strains in china. Then the synthesized peptides were conjugated to carrier protein. The antigenicity of the artificial antigen was identified by dot-blot assay. Based on the BSA-Pep antigen, a test strip was developed to evaluate the neutralizing antibody of serum samples from swine vaccinated with FMDV type O vaccine rapidly. The BSA-Pep used as a detector was labeled with colloidal gold. Staphylococcal protein A (SPA) and anti FMDV antibodies were blotted onto the nitrocellulose membrane as the test and control lines, respectively. The strip assay could be performed within 5 min, which did not require any special equipment or skills. Through testing sera against various strains of FMDV type O, the sensitivity of the strip was determined to be 95% (38/40). There were no cross reactions to other virus antibodies, and no cross reactions in different serotypes of FMDV, the test strip can be used to detect FMDV type O antibodies only. The strip results were consistent with those of the existing commercial ELISA kits. In conclusion, the new strip was an acceptable method for surveying FMDV type O antibody titers in pigs.
机译：根据我国主要的FMDV O型疫苗株设计合成了四种肽。然后将合成的肽与载体蛋白缀合。人工抗原的抗原性通过斑点印迹法鉴定。基于BSA-Pep抗原，开发了一种试纸，用于快速评估接种FMDV O型疫苗的猪血清样品的中和抗体。用作检测器的BSA-Pep用胶体金标记。将葡萄球菌蛋白A（SPA）和抗FMDV抗体分别印迹到硝酸纤维素膜上，作为测试和对照品系。试纸条检测可在5分钟内完成，不需要任何特殊设备或技能。通过针对各种FMDV O型菌株测试血清，检测条的灵敏度为95％（38/40）。与其他病毒抗体没有交叉反应，在不同血清型的FMDV中也没有交叉反应，试纸只能用于检测FMDV O型抗体。试纸条结果与现有商业ELISA试剂盒的结果一致。总而言之，新的检测条是用于调查猪FMDV O型抗体滴度的可接受方法。

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