Immune response effects of diverse vaccine antigen attachment ways based on the self-made nanoemulsion adjuvant in systemic MRSA infection

Liu-yang Yang; Chao Wei; Yun Yang; Ya-nan Tong; Sha Yang; Liu-sheng Peng; Qian-fei Zuo; Yuan Zhuang; Ping Cheng; Hao Zeng; Quan-ming Zou; Hong-wu Sun

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Immune response effects of diverse vaccine antigen attachment ways based on the self-made nanoemulsion adjuvant in systemic MRSA infection

机译：基于自制纳米乳佐剂的多种疫苗抗原附着方式对全身MRSA感染的免疫应答作用

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Nanoemulsion adjuvants-based vaccines have potent induced immune responses against methicillin-resistant Staphylococcus aureus (MRSA) infection. However, the efficacies and immune responses of different antigen-attaching ways on self-made nanoemulsion adjuvants remain unknown. In this study, we designed three formulations of nanoemulsion adjuvants (encapsulation, mixture, and combination) to explore their immune response-enhancing effects and their underlying mechanism in a systemic infection model of MRSA. Our results showed that the three nanoemulsion-attachment ways formulated with a fusion antigen of MRSA (Hla _(H35L) IsdB _(348–465) ) all improved humoral and cellular immune responses. When compared with the mixture and combination formulations, the nanoemulsion-encapsulation group effectively promoted the antigen uptake of dendritic cells (DCs) in vitro , the activation of DC in draining lymph nodes and the delayed release of antigen at injection sites in vivo . Moreover, the encapsulation group induced a more ideal protective efficacy in a MRSA sepsis model by inducing more potent antibody responses and a Th1/Th17 biased CD4 ~(+) T cell response when compared with the other two attachment ways. Our findings suggested that the encapsulated formulation of vaccine with nanoemulsion adjuvant is an effective attachment way to provide protective immunity against MRSA infection.

机译：基于纳米乳液佐剂的疫苗对耐甲氧西林的金黄色葡萄球菌（MRSA）感染具有有效的诱导免疫反应。然而，不同的抗原附着方式对自制纳米乳液佐剂的功效和免疫应答仍然未知。在这项研究中，我们设计了三种纳米乳剂佐剂（封装，混合和组合），以探讨它们在MRSA系统感染模型中的免疫应答增强作用及其潜在机制。我们的结果表明，使用MRSA融合抗原（Hla _（H35L）IsdB _（348-465））配制的三种纳米乳剂附着方法均改善了体液和细胞免疫反应。当与混合物和组合制剂相比时，纳米乳液包封组在体外有效促进树突状细胞（DC）的抗原摄取，引流淋巴结中DC的活化以及体内注射部位抗原的延迟释放。此外，与其他两种附着方式相比，封装组通过诱导更有效的抗体反应和Th1 / Th17偏向的CD4〜（+）T细胞反应，在MRSA脓毒症模型中诱导了更理想的保护效果。我们的研究结果表明，纳米乳佐剂的疫苗包封制剂是一种有效的附着方式，可提供针对MRSA感染的保护性免疫。

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《RSC Advances》 |2018年第19期|共12页
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Liu-yang Yang; Chao Wei; Yun Yang; Ya-nan Tong; Sha Yang; Liu-sheng Peng; Qian-fei Zuo; Yuan Zhuang; Ping Cheng; Hao Zeng; Quan-ming Zou; Hong-wu Sun;
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1. Immune response effects of diverse vaccine antigen attachment ways based on the self-made nanoemulsion adjuvant in systemic MRSA infection [J] . Yang Liu-yang, Wei Chao, Yang Yun, RSC Advances . 2018,第19期

机译：基于自制造纳米乳液佐剂在全身MRSA感染中不同疫苗抗原附着方式的免疫反应影响
2. Buccal injection of synthetic HPV long peptide vaccine induces local and systemic antigen-specific CD8+ T-cell immune responses and antitumor effects without adjuvant [J] . Ming-Chieh Yang, Andrew Yang, Jin Qiu, Cell & Bioscience . 2016,第1期

机译：口腔内注射合成的HPV长肽疫苗可诱导局部和全身性抗原特异性CD8 + T细胞免疫反应，并在无佐剂的情况下具有抗肿瘤作用
3. Induction of systemic and mucosal immunity against methicillin-resistant Staphylococcus aureus infection by a novel nanoemulsion adjuvant vaccine [J] . HongWu Sun, Chao Wei, BaoShuai Liu, International Journal of Nanomedicine . 2015,第1期

机译：新型纳米乳佐剂疫苗诱导对耐甲氧西林的金黄色葡萄球菌感染的全身和粘膜免疫

4. PROTEIN AND ADJUVANT CO-ENCAPSULATED IN PLG MICROSPHERES ELICIT A STRONG COMPREHENSIVE IMMUNE RESPONSE AGAINST A M. TUBERCULOSIS ANTIGEN [C] . J.T. Evans, J.R. Ward, J. Kern, Proceedings of the 29th Annual Meeting of the Controlled Release Society . 2002

机译：蛋白和佐剂共包被在PLG微球中，可引起针对结核分枝杆菌抗原的强力综合免疫反应

5. Potentiation of the immune response with aluminum-containing adjuvants: I. Distribution of adsorbed antigen in mono-valent and combination vaccines; II. Role of antigen adsorption in antigen internalization by dendritic cells. [D] . Morefield, Garry Lee, Jr. 2003

机译：含铝佐剂增强免疫应答：I.单价和组合疫苗中吸附抗原的分布；二。抗原吸附在树突状细胞的抗原内在化中的作用。

6. Buccal injection of synthetic HPV long peptide vaccine induces local and systemic antigen-specific CD8+ T-cell immune responses and antitumor effects without adjuvant [O] . Ming-Chieh Yang, Andrew Yang, Jin Qiu, 2016

机译：口腔注射合成的HPV长肽疫苗可诱导局部和全身性抗原特异性CD8 + T细胞免疫反应并在无佐剂的情况下具有抗肿瘤作用

7. Buccal injection of synthetic HPV long peptide vaccine induces local and systemic antigen-specific CD8+ T-cell immune responses and antitumor effects without adjuvant [O] . Ming-Chieh Yang, Andrew Yang, Jin Qiu, 2016

机译：口腔内注射合成的HPV长肽疫苗可诱导局部和全身性抗原特异性CD8 + T细胞免疫反应，并在无佐剂的情况下具有抗肿瘤作用

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Immune response effects of diverse vaccine antigen attachment ways based on the self-made nanoemulsion adjuvant in systemic MRSA infection

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