Extracellular pH imaging of a plant leaf with a polyelectrolyte multilayered nanosheet

Daichi Someya; Satoshi Arai; Toshinori Fujie; Shinji Takeoka

首页> 外文期刊>RSC Advances >Extracellular pH imaging of a plant leaf with a polyelectrolyte multilayered nanosheet

【24h】

Extracellular pH imaging of a plant leaf with a polyelectrolyte multilayered nanosheet

机译：带有聚电解质多层纳米片的植物叶片的细胞外pH成像

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We have developed a sheet-like pH imaging sensor based on a flexible and physically adhesive polymer thin film (referred to as a “pH sensing nanosheet”). The pH sensing nanosheet was composed of two films: one is a pH-sensitive layer-by-layer (LbL) film constructed from fluorescein-conjugated poly(acrylic acid) and poly(allylamine hydrochloride) and the other is a pH-insensitive film made from Nile red-embedded poly( D , L -lactic acid). The pH sensing nanosheet enabled the ratiometric imaging of pH changes in a leaf (500 × 500 μm ~(2) ), namely the apoplastic ion milieu responding to an external NaCl stress. It was successfully mapped out that the alkalization of the leaf apoplast spread from the leaf base to the tip at 20 min after the stimulation and the pH value increased up to approximately pH 6.3 from less than pH 4.5 within 60 min when a 100 mM NaCl aqueous solution was added. The pH sensing nanosheet should be useful for energy metabolic mapping in tissue biology.

机译：我们已经开发了一种基于柔性且物理粘合的聚合物薄膜的片状pH成像传感器（称为“ pH传感纳米片”）。 pH敏感纳米片由两层膜组成：一层是由荧光素共轭的聚丙烯酸和聚烯丙胺盐酸盐构成的pH敏感层（LbL）膜，另一层是pH敏感膜由尼罗河红包埋的聚（D，L-乳酸）制成。通过pH传感纳米片，可以对叶子（500×500μm〜（2））中的pH变化进行比例成像，即响应外部NaCl胁迫的质外离子环境。成功绘制出在刺激后20分钟时叶质外体的碱化作用从叶基向叶尖蔓延，当使用100 mM NaCl水溶液时，pH值在60分钟内从低于pH 4.5升高到大约pH 6.3。解决方案已添加。 pH传感纳米片应可用于组织生物学中的能量代谢作图。

著录项

来源
《RSC Advances》 |2018年第62期|共7页
作者
Daichi Someya; Satoshi Arai; Toshinori Fujie; Shinji Takeoka;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类化学;
关键词

相似文献

外文文献
中文文献
专利

1. Extracellular pH imaging of a plant leaf with a polyelectrolyte multilayered nanosheet [J] . Someya Daichi, Arai Satoshi, Fujie Toshinori, RSC Advances . 2018,第62期

机译：具有聚电解质多层纳米液的植物叶的细胞外pH成像
2. Extracellular pH imaging of a plant leaf with a polyelectrolyte multilayered nanosheet [J] . Daichi Someya, Satoshi Arai, Toshinori Fujie, RSC Advances . 2018,第62期

机译：带有聚电解质多层纳米片的植物叶片的细胞外pH成像
3. Live imaging of intra- and extracellular pH in plants using pHusion, a novel genetically encoded biosensor [J] . Gjetting Kisten Sisse Krag, Ytting Cecilie Karkov, Schulz Alexander, Journal of Experimental Botany . 2012,第8期

机译：使用pHusion（一种新型的遗传编码生物传感器）对植物体内和细胞外pH进行实时成像
4. Leaf-Based Plant Identification Through Morphological Characterization in Digital Images [C] . Arturo Oncevay-Marcos, Ronald Juarez-Chambi, Sofia Khlebnikov-Nunez, International conference on computer analysis of images and patterns . 2015

机译：通过数字图像的形态表征识别基于叶的植物
5. Physical characterization of erodible multilayered polyelectrolyte thin films fabricated from plasmid DNA and synthetic polyamines. [D] . Fredin, Nathaniel Jon. 2008

机译：由质粒DNA和合成多胺制备的易蚀多层聚电解质薄膜的物理表征。
6. Live imaging of intra- and extracellular pH in plants using pHusion a novel genetically encoded biosensor [O] . Kisten Sisse Krag Gjetting, Cecilie Karkov Ytting, Alexander Schulz, -1

机译：使用pHusion（一种新型的遗传编码生物传感器）对植物体内和细胞外pH进行实时成像
7. P1246Age-, body size-, and sex-specific reference values for tricuspid valve apparatus parameters by real-time three-dimensional transthoracic echocardiographyP1247Surgical therapy in infective endocarditis: predisposing and prognostic factorsP1248Evalutation of MV annulus geometry modifications before and after mitraclip implantation with 3D transesophageal echocardiographyP1249Prognostic echocardiographic parameters for successful transcatheter edge-to-edge mitral valve repairP1250Influence of body surface area in diagnosis and outcome patient-prosthesis mismatch after TAVI. Is it necessary to adjust for ideal body weight?P1251Outcome of paradoxical low-flow low-gradient severe AS following TAVI: paradoxical VS parallel to outcome of high gradient ASP1252Prognostic role of cardiac calcifications in subjects without overt significant heart disease and previous cardiovascular events: is there an additive value over cardiovascular risk factors scores?P1253Aging degeneration of mitral valve without leaflet prolapse or tethering as a cause of severe mitral regurgitationP1254Cardiovascular risk assessment should be performed in patients with incidental aortic sclerosisP1255Cardiac valve calcification score: simple 2D echocardiographic tool for cardiovascular risk stratification in patients with chronic renal failureP1256Diastolic mitral regurgitation following transcatheter aortic valve replacement: prevalence, predictors and impact on outcomesP1257Timing of myocardial shortening determines left ventricular regional remodelling in hearts with conduction delaysP1258Myocardial stiffness assessment using shear wave imaging in healthy children and hypertrophic cardiomyopathyP1259Assessment of right ventricular response to exercise using vector velocity imaging in hypertrophic cardiomyopathyP1260Diagnostic value of cardiac biomarkers (high sensitive troponin T and N-terminal pro-brain natriuretic peptide) in patients with infiltrative cardiomyopathiesP1261Right ventricular global longitudinal strain differentiates between adolescent patients with definite, borderline and possible ARVC and controlsP1262 echogrqphic predictif factors of atrial arrhythmias in patients with arrhythmogenic right ventricular dysplasiaP1263Importance of combined left atrial size and estimated pulmonary pressure for clinical outcome in patients presenting with heart failure with preserved ejection fractionP1264Chronotropic response in mitochondrial (ANT1) cardiomyopathyP1265 The effect of cardiovascular risk factors and cancer type in anthracycline's cardiotoxicityP1266Revisiting the echocardiographic features of acute myocarditisP1267Altered circulation in fetuses of diabetic mothers: a fetal computational model analysisP1268Increased epicardial adipose tissue is related to stress-induced cardiomyopathyP1269Contrast enhanced ultrasound of kidney perfusion in patients with renal artery stenosis: diagnostic and prognostic applications. [O] . F. Ancona, R. Ilhao Moreira, V. Lavanco, 2016

机译：P1246age-，身体大小和性别特异性参考Tricuspid阀装置参数通过实时三维经线性超声心动图124772777777747774747478EVALIGUTALMEXP1249预期植入前后MV环形植入前后MV环空性修饰。超声心动图参数用于成功的经截管边缘到边缘二尖瓣修复P1250中的体表面积诊断和结果患者假体在Tavi后的不匹配。是否有必要调整理想的体重？P1251矛盾的低流量低梯度严重，如Tavi：矛盾的VS平行于高梯度ASP1252的结果，在没有明显显着的心脏病和以前心血管事件的情况下心脏钙化的促傲作用患有心血管危险因素的添加剂值分数？没有传单脱垂的二尖瓣退化或作为严重二尖瓣血管血管血管病风险评估的患者进行脱垂或系绳，应当在偶然主动脉源肺源P1255Cariac阀钙化评分患者中进行：用于心血管风险分层的简单2D超声心动图工具在慢性肾功能衰竭的患者中，经沟管主动脉瓣膜置换术后：流行率，预测因子和对Otcomasp1257的影响，心肌缩短决定了导通延迟的心中的剩余心室区域重新耦合258MYCIARIAL僵硬评估使用剪切波成像在健康儿童和肥厚性心肌脑病中的舒缓性心脏响应，在渗透心肌肌瘤患者中使用载体速度成像的培养速度成像（高敏感的肌钙蛋白T和N-末端脑Natrietic肽）渗透心肌病，患者心室全局纵向菌株在患有心律失常右心室发育不良患者的心理心律失常患者中的青少年患者与Controlsp1262 echogrqphic预测因子的分歧，患者心血性右心室发育不良患者患者患者患者患者患者患者患者患者患者线粒体（ANT1）心肌病患者的喷射FRACTIONP1264系列响应1265心血管危险因素和癌症类型在蒽丙烯的心肌毒性皮1126660中的影响访问患有糖尿病母亲胎儿胎儿急性心肌炎的超声心动图，患者患者：胎儿计算模型分析P1268增加外膜脂肪组织与肾动脉狭窄患者肾脏灌注的应激诱导的心肌病，增强超声波：诊断和预后应用。

Extracellular pH imaging of a plant leaf with a polyelectrolyte multilayered nanosheet

摘要

著录项

相似文献

相关主题

期刊订阅