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首页> 外文期刊>RSC Advances >A simple, fast, and sensitive colorimetric assay for visual detection of berberine in human plasma by NaHSO4-optimized gold nanoparticles
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A simple, fast, and sensitive colorimetric assay for visual detection of berberine in human plasma by NaHSO4-optimized gold nanoparticles

机译：NaHSO 4 优化的金纳米粒子用于视觉检测人血浆中小ber碱的简单，快速，灵敏的比色测定

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摘要

Berberine is an important anti-inflammatory drug widely used for the treatment of heart and intestinal disorders. The quantification of berberine is usually required in clinic medical assays. Herein, we report simple, fast and sensitive colorimetric visualization of berberine in human plasma using citrate-stabilized gold nanoparticles (AuNPs). Upon exposure to μg mL?1 levels of berberine, the AuNP solution exhibits a highly sensitive color change from red to blue and rapid aggregation kinetics within the initial 10 min, which can be directly seen with the naked eye and monitored by UV-vis absorbance spectroscopy. As confirmed by comparison with potential coexisting foreign substances, the presence of positively charged berberine induces the aggregation of the AuNPs owing to the electrostatic attraction and coordination interactions between the ring nitrogen atoms of berberine and the AuNPs, causing a color change of the AuNP suspension. Further evidence is that the sensitivity is significantly improved when NaHSO4 is added to promote the ligand exchange between citrate and berberine at the surface of the AuNPs. The NaHSO4-optimized AuNP system provides a simple colorimetric assay for the rapid detection of berberine in human plasma. The limit of detection (LOD) is 0.24 μg mL?1 by the naked eye and 0.06 μg mL?1 by UV-vis spectroscopy.
机译：小ber碱是一种重要的抗炎药，广泛用于治疗心脏和肠道疾病。临床医学测定中通常需要对小ber碱进行定量。本文中，我们报告了使用柠檬酸盐稳定的金纳米颗粒（AuNPs）在人血浆中对小ber碱进行简单，快速和灵敏的比色可视化。暴露于μgmL ？1 小ber碱水平时，AuNP溶液在最初的10分钟内显示出从红色到蓝色的高度敏感的颜色变化以及快速的聚集动力学，这可以可以直接用肉眼看到，并通过紫外可见吸收光谱法进行监控。通过与潜在共存的异物比较，证实了，由于小ber碱和AuNPs的环氮原子之间的静电吸引和配位相互作用，带正电荷的小ber碱会诱导AuNPs聚集，从而引起AuNP悬浮液的颜色变化。进一步的证据表明，添加NaHSO 4 来促进AuNPs表面柠檬酸和小ber碱之间的配体交换，可以显着提高灵敏度。 NaHSO 4 优化的AuNP系统提供了一种简单的比色测定法，用于快速检测人血浆中的小assay碱。肉眼检测限（LOD）为0.24μgmL ？1 和0.06μgmL ？1 通过紫外可见光谱。

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