Copolymerization of Eu(TTA)<sub>3</sub>Phen doped styrene and methyl methacrylate nanoparticles and use in quantitative detection of pepsinogen

Feng Wu; Mao Mao; Yu Cen; Hongtian Yang; Zhifeng Qin; Lan Ma

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Copolymerization of Eu(TTA)₃Phen doped styrene and methyl methacrylate nanoparticles and use in quantitative detection of pepsinogen

机译：Eu（TTA）_{3 苯掺杂的苯乙烯和甲基丙烯酸甲酯纳米粒子的共聚及其在胃蛋白酶原定量检测中的应用}

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High fluorescence intensity nanoparticles were prepared by copolymerization of Eu(TTA)₃Phen doped styrene and methyl methacrylate. The fluorescent nanoparticles were used as labels for the quantitative detection of serum pepsinogens. Anti-pepsinogen I antibodies and anti-pepsinogen II antibodies were coated on the nitrocellulose membrane as test line 1 and test line 2 respectively. Serum pepsinogen I and pepsinogen II were detected simultaneously in one strip through sandwich immunoassay. This immunoassay strip can analyze the serum pepsinogens in one step and obtain results within 15 minutes. The limit of detection of this immunoassay strip for PG I and PG II standard analytes was 0.5 ng mL?1. The reproducibility and recovery tests shows that the quantitative results were accurate, and the strip can be used in clinical applications.

机译：通过Eu（TTA） _{3 Phen掺杂的苯乙烯和甲基丙烯酸甲酯的共聚制备了高荧光强度的纳米粒子。荧光纳米颗粒用作定量检测血清胃蛋白酶原的标记。将抗胃蛋白酶原I抗体和抗胃蛋白酶原II抗体分别涂在硝酸纤维素膜上作为测试线1和测试线2。通过夹心免疫测定法同时检测一条血清中的胃蛋白酶原I和胃蛋白酶原II。该免疫分析条可一步分析血清胃蛋白酶原，并在15分钟内获得结果。免疫分析条的PG I和PG II标准分析物的检出限为0.5 ng mL ？1 。再现性和回收率测试表明定量结果是准确的，该胶条可用于临床。}

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《RSC Advances》 |2017年第20期|共7页
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Feng Wu; Mao Mao; Yu Cen; Hongtian Yang; Zhifeng Qin; Lan Ma;
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1. Copolymerization of Eu(TTA)₃Phen doped styrene and methyl methacrylate nanoparticles and use in quantitative detection of pepsinogen [J] . Feng Wu, Mao Mao, Yu Cen, RSC Advances . 2017,第20期

机译：Eu（TTA）_{3 苯掺杂的苯乙烯和甲基丙烯酸甲酯纳米粒子的共聚及其在胃蛋白酶原定量检测中的应用}
2. Free-radical copolymerization of methyl methacrylate with styrene in the presence of 2-mercaptoethanol II. Influence of methyl methacrylate/styrene ratio [J] . Teodorescu M. European Polymer Journal . 2002,第5期

机译：在2-巯基乙醇II的存在下，甲基丙烯酸甲酯与苯乙烯的自由基共聚。甲基丙烯酸甲酯/苯乙烯比率的影响
3. Determination of the Radical Reactivity Ratios of 2-(N-Ethylperfluorooctanesulfonamido)ethyl Acrylate and Methacrylate in Copolymerizations with N,N-Dimethylacrylamide by in Situ H-1 NMR Analysis As Established for Styrene-Methyl Methacrylate Copolymerizations [J] . Debnath Dibyendu, Baughman Jessi A., Datta Sujay, Macromolecules . 2018,第20期

机译：用苯乙烯 - 甲基丙烯酸甲酯共聚成立，用N，N-二甲基丙烯酰胺与N，N-二甲基丙烯酰胺与N，N-二甲基丙烯酰胺中的2-（N-乙基氟辛酰磺酰胺酰胺和甲基丙烯酸甲酯的自由基反应比。
4. Graft Copolymerization of Methyl Methacrylate From Poly(Glycidyl Methacrylate-co-Styrene) Initiated by Cp2TiCl-Catalyzed Epoxide Radical Ring Opening [C] . Alexandru D. Asandei, Gobinda Saha PMSE Symposia . 2006

机译：CP2TICL催化环氧化物 - 开口引发的聚（甲基丙烯酸甲酯 - 共苯乙烯）从甲基丙烯酸甲酯的接枝共聚
5. Copolymerization of ethyl acrylate and methyl methacrylate in the presence of isopropyl(pyridinato)cobaloxime. [D] . Huang, Chung-Yu. 2000

机译：在异丙基（吡啶并）钴肟的存在下丙烯酸乙酯和甲基丙烯酸甲酯的共聚。
6. A new initiating system based on (SiMes)Ru(PPh3)(Ind)Cl2 combined with azo-bis-isobutyronitrile in the polymerization and copolymerization of styrene and methyl methacrylate [O] . Abdullah M. Al-Majid, Waseem Sharaf Shamsan, Abdel-Basit Mohammed Al-Odayn, 2017

机译：SiMes Ru（PPh3）（Ind）Cl2与偶氮-双-异丁腈结合的新型引发体系用于苯乙烯和甲基丙烯酸甲酯的聚合和共聚
7. Copolymerization of Eu(TTA)3Phen doped styrene and methyl methacrylate nanoparticles and use in quantitative detection of pepsinogen [O] . Feng Wu, Mao Mao, Yu Cen, 2017

机译：Eu（TTA）3Phen掺杂苯乙烯和甲基丙烯酸甲酯纳米粒子的共聚，用途胃蛋白酶原定量检测
8. Organophosphazenes.22 Copolymerization of (Alpha-Methylethenylphenyl) Penta-fluorocyclotriphosphazenes with Styrene and Methyl Methacrylate [R] . Allen, C. W., Shaw, J. C., Brown end, D. E. 1988

机译：有机磷腈.22（α-甲基苯基苯基）五氟环三磷腈与苯乙烯和甲基丙烯酸甲酯的共聚合

Copolymerization of Eu(TTA)3Phen doped styrene and methyl methacrylate nanoparticles and use in quantitative detection of pepsinogen

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Copolymerization of Eu(TTA)₃Phen doped styrene and methyl methacrylate nanoparticles and use in quantitative detection of pepsinogen