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首页> 外文期刊>RSC Advances >Preliminary characterization of the structure and immunostimulatory and anti-aging properties of the polysaccharide fraction of Haematococcus pluvialis
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Preliminary characterization of the structure and immunostimulatory and anti-aging properties of the polysaccharide fraction of Haematococcus pluvialis

机译：雨生红球菌多糖部分的结构，免疫刺激和抗衰老特性的初步表征

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In this study, a polysaccharide fraction (HPP-c3-s1) was obtained from Haematococcus pluvialis using DEAE-52 anion exchange and Sephacryl S400 chromatographies. The structure of HPP-c3-s1 was partially characterized and its biological activity was investigated. HPP-c3-s1 is a homogeneous polysaccharide with a molecular weight of 23?413 kDa as determined by high-performance gel permeation chromatography and polyacrylamide gel electrophoresis. Periodate oxidation and Smith degradation analysis combined with GC-MS determined that HPP-c3-s1 contains 1 → 2, 1 → 3, 1 → 4, and probably a few 1 → 6 glycosyl linkages. Fourier transform infrared spectroscopy and nuclear magnetic resonance analyses indicate HPP-c3-s1 is a pyranose containing an amino and O-acetyl group. The sugar chains in HPP-c3-s1 contain both α and β glycosidic configurations, where the β configuration is the primary form. It was observed that the HPP-c3-s1 nanostructure had linear and branched forms at a concentration of 5 μg mL ~(?1) and created different sized aggregates at a higher concentration of 50 μg mL ~(?1) . Furthermore, HPP-c3-s1 had significant immunostimulatory effects on splenocytes and B lymphocytes. In assays assessing anti-aging effects, HPP-c3-s1 extended the mean survival of Caenorhabditis elegans without adversely affecting reproduction. In addition, treatment with HPP-c3-s1 resulted in delays in age-related physiologic parameters, including body movement, rates of head swing and body bending, and accumulation of intestinal lipofuscinosis in C. elegans . Overall, these results suggest HPP-c3-s1 has remarkable immunomodulatory and anti-aging properties that may serve as the basis for development of functional foods and dietary supplements.
机译：在这项研究中，使用DEAE-52阴离子交换和Sephacryl S400色谱法从雨生红球菌获得了多糖级分（HPP-c3-s1）。 HPP-c3-s1的结构已部分表征，并对其生物学活性进行了研究。 HPP-c3-s1是通过高效凝胶渗透色谱法和聚丙烯酰胺凝胶电泳测定的分子量为23？413 kDa的均质多糖。高碘酸盐氧化和Smith降解分析与GC-MS相结合，确定HPP-c3-s1包含1→2，1→3、1→4，并可能包含1→6个糖基键。傅里叶变换红外光谱和核磁共振分析表明，HPP-c3-s1是含有氨基和O-乙酰基的吡喃糖。 HPP-c3-s1中的糖链同时包含α和β糖苷构型，其中β构型是主要形式。观察到，HPP-c3-s1纳米结构在浓度为5μgmL〜（？1）时呈线性和支链形式，并在浓度为50μgmL〜（？1）时产生不同尺寸的聚集体。此外，HPP-c3-s1对脾细胞和B淋巴细胞具有明显的免疫刺激作用。在评估抗衰老作用的测定中，HPP-c3-s1延长了秀丽隐杆线虫的平均生存期，而没有对繁殖造成不利影响。另外，用HPP-c3-s1治疗导致与年龄相关的生理参数的延迟，包括身体运动，头部摆动和身体弯曲的速率以及秀丽隐杆线虫中肠脂褐藻病的积累。总体而言，这些结果表明HPP-c3-s1具有显着的免疫调节和抗衰老特性，可作为开发功能性食品和膳食补充剂的基础。

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