• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Isolation, differentiation and biodiversity of ureolytic bacteria of Qatari soil and their potential in microbially induced calcite precipitation (MICP) for soil stabilization
【24h】

Isolation, differentiation and biodiversity of ureolytic bacteria of Qatari soil and their potential in microbially induced calcite precipitation (MICP) for soil stabilization

机译：卡塔尔土壤中溶尿细菌的分离，分化和生物多样性及其在微生物诱导的方解石沉淀（MICP）中用于土壤稳定的潜力

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Biomineralization plays a key role in modifying the geological properties of soil, thereby stabilizing it against wind erosion, especially in areas characterized by harsh weather and harsh soil (calcareous and arid); i.e. Arabic Gulf region. Among soil microorganisms, ureolytic bacteria are capable of modifying soil characteristics and thus, inducing biomineralization. This research investigated the occurrence and diversity of ureolytic bacteria in Qatari soils, specifically to study their acquired potential to adapt to harsh conditions exhibiting ureolytic activity. Soil samples were collected from various locations in Qatar and were used to isolate the indigenous ureolytic bacteria. It was noticed that most of the ureolytic bacteria in Qatari soil belong to the genus Bacillus mainly Bacillus cereus . Identification and differentiation of 18 ureolytic isolates were performed using MALDI-TOF MS techniques while ribotyping (16S rRNA) molecular technique was used mainly for 6 selected strains. This study not only shows the diversity of species of ureolytic bacteria in Qatari soil but also shows the diversity in their protein profiles, which confirms that bacteria have adapted well to the harsh environment. In addition, the strains were evaluated based on a newly modified screening method in this work; i.e. production of arbitrary urease activity (AUA). Thus, the strains showing the highest AUA, exhibited the highest capability to produce urease enzymes induced by urea. Analysis of calcium carbonate precipitation utilizing SEM-EDX showed that the ureolytic bacteria also play a significant role in the precipitation of minerals such as CaCO _(3) , in the presence of urea in soil. Therefore, this research showed a high occurrence of indigenous Bacillus bacteria in Qatari soil that can perform biomineralization and thus can be helpful, if properly stimulated, in enhancing soil stabilization, and for other local applications as well, since they are adapted to these soil and weather conditions.
机译：生物矿化在改变土壤的地质特性，从而稳定其抵抗风蚀方面起着关键作用，特别是在恶劣的天气和恶劣的土壤（钙质和干旱）地区;即阿拉伯湾地区。在土壤微生物中，尿素分解细菌能够改变土壤特性，从而诱导生物矿化。这项研究调查了卡塔尔土壤中尿素分解细菌的发生和多样性，特别是研究了其适应显示尿素分解活性的苛刻条件的潜力。从卡塔尔各地收集土壤样品，并将其用于分离本地的溶尿细菌。注意到卡塔尔土壤中的大多数溶尿细菌属于芽孢杆菌属，主要是蜡状芽孢杆菌。使用MALDI-TOF MS技术进行了18种尿素分解菌株的鉴定和分化，而核糖分型（16S rRNA）分子技术则主要用于6个所选菌株。这项研究不仅显示了卡塔尔土壤中溶尿细菌的种类多样性，还显示了其蛋白质谱的多样性，这表明细菌已经很好地适应了恶劣的环境。此外，在这项工作中，基于一种新的筛选方法对菌株进行了评估。即产生任意的脲酶活性（AUA）。因此，显示最高AUA的菌株表现出产生由尿素诱导的脲酶的最高能力。利用SEM-EDX分析碳酸钙沉淀表明，在土壤中存在尿素的情况下，尿素分解细菌在诸如CaCO _（3）等矿物质的沉淀中也起着重要作用。因此，这项研究表明，卡塔尔土壤中存在大量的本地芽孢杆菌细菌，这些细菌可以进行生物矿化处理，因此，如果受到适当刺激，则可以增强土壤稳定性，并有助于其他方面的应用，因为它们适用于这些土壤和土壤。天气状况。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号