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首页> 外文期刊>RSC Advances >Green synthesis, physio-chemical characterization and anti-candidal function of a biocompatible chitosan gold nanocomposite as a promising antifungal therapeutic agent
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Green synthesis, physio-chemical characterization and anti-candidal function of a biocompatible chitosan gold nanocomposite as a promising antifungal therapeutic agent

机译：生物相容性壳聚糖金纳米复合材料作为有前景的抗真菌治疗剂的绿色合成，理化特性和抗候选功能

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A chitosan gold nanocomposite (CAuNC) was green synthesized using chitosan and gold(III) chloride trihydrate without using a reducing agent. The formation and crystalline nature of the gold nanoparticles (AuNPs) in CAuNC were confirmed by UV-visible spectrum and XRD analysis. The average particle size and zeta potential of CAuNC were 308 nm and +37.45 mV, respectively. The CAuNC contains 13.56% (w/w) of Au, and released 8.56 ppb Au ions into potato dextrose broth (PDB). The CAuNC was evaluated for its antifungal activities using Candida albicans as a model organism, and the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were recorded as 50 and 75 μg mL?1, respectively. Propidium iodide (PI) uptake and FE-SEM analysis results suggest that CAuNC enhances the permeability and structural changes of C. albicans cells in a concentration dependent manner. Exposure of CAuNC increased the mitochondrial membrane potential, and dysfunctions of mitochondria could induce the reactive oxygen species (ROS) level and increase the oxidative stress. Moreover, the expression of some C. albicans proteins has decreased after CAuNC treatment. The CAuNC was nontoxic to mammalian A549 and HEK293T cells up to 100 μg mL?1 suggesting that it can be applied to in vivo tests. C. albicans infected zebrafish recovered after CAuNC therapy as a topical treatment, suggesting that CAuNC is a potential antifungal agent against candidiasis. This report illustrates the eco-friendly approach of synthesizing biologically active CAuNC as a potent antifungal agent against C. albicans.
机译：壳聚糖和三氯化金（ III ）三水合物在不使用还原剂的情况下绿色合成了壳聚糖金纳米复合材料（CAuNC）。通过紫外可见光谱和XRD分析证实了CAuNC中金纳米颗粒（AuNPs）的形成和晶体性质。 CAuNC的平均粒径和Zeta电位分别为308 nm和+37.45 mV。 CAuNC含有13.56％（w / w）的Au，并向土豆葡萄糖肉汤（PDB）中释放了8.56 ppb的Au离子。以白色念珠菌为模型生物评估了CAuNC的抗真菌活性，记录的最小抑菌浓度（MIC）和最小杀真菌浓度（MFC）为50和75μgmL < sup>？1 。碘化丙啶（PI）的摄取和FE-SEM分析结果表明，CAuNC增强了C的渗透性和结构变化。白色念珠菌细胞的浓度依赖性。暴露于CAuNC会增加线粒体膜电位，而线粒体功能障碍会诱导活性氧（ROS）水平并增加氧化应激。而且，某些 C的表达。 CAuNC处理后，白色念珠菌蛋白降低。 CAuNC对高达100μgmL ？1 的哺乳动物A549和HEK293T细胞无毒，这表明它可以用于体内测试。 C。 CAuNC作为局部治疗后，恢复了白色念珠菌感染的斑马鱼，这表明CAuNC是潜在的抗念珠菌病药物。该报告说明了合成具有生物活性的CAuNC作为对 C的有效抗真菌剂的生态友好方法。白色的。

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