Electroactive scaffolds which can carry electrical stimulation to the cells growing on them have attracted more and more attention in recent years. In this study, a conductive fibrous scaffold made of silk fibroin (SF) and graphene was developed using electrospinning techniques. The chemical structural characterization of the obtained scaffolds confirmed the presence of graphene in the fibrous scaffolds. The surface morphologies, mechanical and electrochemical properties and cytocompatibility of the scaffolds were evaluated. The average diameters of the G/SF fibrous scaffolds increased with the addition of graphene until the content of graphene reached 4%. The G/SF scaffolds exhibited improved thermal stability with the addition of graphene, which confirmed that they were more crystalline than pure SF scaffolds. The 3% G/SF fibrous scaffolds showed improved electroactivity and mechanical properties. In addition, they could support the growth and expansion of rat bone mesenchymal stem cells (rBMSCs) based on cell morphology, viability and proliferation studies in vitro. Thus, all the data in this study suggested that the 3% G/SF scaffolds might represent an adequate substrate to successfully scaffold electroactive tissue during regeneration or engineering.
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机译：近年来，能够对在其上生长的细胞进行电刺激的电活性支架受到越来越多的关注。在这项研究中，使用静电纺丝技术开发了由丝素蛋白（SF）和石墨烯制成的导电纤维支架。获得的支架的化学结构表征证实了在纤维支架中存在石墨烯。评价了支架的表面形态，机械和电化学性质以及细胞相容性。 G / SF纤维支架的平均直径随着石墨烯的添加而增加，直到石墨烯的含量达到4％。 G / SF支架通过添加石墨烯表现出改善的热稳定性，这证实了它们比纯SF支架更具结晶性。 3％G / SF纤维支架显示出改善的电活性和机械性能。此外，它们可以通过体外的细胞形态，活力和增殖研究，支持大鼠骨间充质干细胞（rBMSC）的生长和扩增。因此，这项研究中的所有数据表明，3％的G / SF支架可能代表了在再生或工程过程中成功支架电活性组织的合适底物。
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