A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick

Sonu Gandhi; Ivneet Banga; Pawan Kumar Maurya; Sergei A. Eremin

首页> 外文期刊>RSC Advances >A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick

【24h】

A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick

机译：金纳米颗粒单链片段可变抗体作为免疫探针，可通过量油尺快速检测吗啡

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Gold nanoparticle (AuNP)-based optical assays are of significant interest since the molecular phenomenon can be examined easily with change in the color of AuNPs. Herein, we report the development of a dipstick using a AuNP-labeled single-chain fragment variable (scFv) antibody for the detection of morphine. The scFv antibodies for morphine were developed using phage display-based antibody library. Immunoglobulin variable regions of heavy (V_H)- and light (V_L)-chain genes were connected via a glycine–serine linker isolated from murine immune repertoire and cloned into the expression vector pIT2. The scFv was produced in Escherichia coli HB2151, yielding a functional protein with a molecular weight of approximately 32 kDa. The morphine scFv was labeled with gold nanoparticles and used as an optical immunoprobe in a dipstick. The competitive dipstick assay characterized the ability of the scFv antibody to recognize free morphine. The detection range was 1–1000 ng mL?1 with a limit of detection (LOD) of 5 ng mL?1 under optimal conditions, and the IC₅₀ value was 14 ng mL?1 for morphine. The developed optical dipstick kit of scFv antibody was capable of specifically binding to free morphine and its analogs in a solution in less than 5 min and could be useful for on-site screening of a real sample in blood, urine, and saliva.

机译：基于金纳米颗粒（AuNP）的光学测定法引起了人们的极大兴趣，因为可以通过改变AuNPs的颜色轻松检查分子现象。在这里，我们报告使用AuNP标记的单链片段可变（scFv）抗体检测吗啡的量油尺的发展。使用基于噬菌体展示的抗体库开发了吗啡的scFv抗体。重（ V _{H ）和轻（ V _{L < / sub> ）链基因通过从小鼠免疫库中分离出来的甘氨酸-丝氨酸接头连接，并克隆到表达载体pIT2中。 scFv在大肠埃希氏菌HB2151中生产，产生分子量约为32 kDa的功能蛋白。吗啡scFv用金纳米颗粒标记，并用作量油尺中的光学免疫探针。竞争量油尺测定法表征了scFv抗体识别游离吗啡的能力。检测范围为1–1000 ng mL ？1 ，检测限（LOD）为5 ng mL ？1 < / small>在最佳条件下，吗啡的IC _{50 值为14 ng mL ？1 。已开发的scFv抗体光学试纸试剂盒能够在不到5分钟的时间内与溶液中的游离吗啡及其类似物特异性结合，可用于现场筛查血液，尿液和唾液中的真实样品。}}} 展开▼

著录项

来源
《RSC Advances》 |2018年第3期|共8页

作者
Sonu Gandhi; Ivneet Banga; Pawan Kumar Maurya; Sergei A. Eremin;
展开▼

作者单位

展开▼

收录信息

原文格式 PDF

正文语种

中图分类化学;

关键词

相似文献

外文文献

中文文献

专利

1. A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick [J] . Gandhi Sonu, Banga Ivneet, Maurya Pawan Kumar, RSC Advances . 2018,第3期

机译：金纳米粒子 - 单链片段可变抗体作为免疫盗，用于通过Dipstick快速检测吗啡

2. Single-chain variable fragment antibody-based immunochromatographic strip for rapid detection of fumonisin B_1 in maize samples [J] . Food Chemistry . 2020,第Jul30期

机译：基于单链可变片段抗体的免疫色谱带可快速检测玉米样品中的伏马菌素B_1

3. Evaluation of a Rapid Immunochromatographic Dipstick Test for Detection of Antibodies to Trypanosoma cruzi in Dogs Experimentally Infected with Isolates Obtained from Opossums (Didelphis virginiana), Armadillos (Dasypus novemcinctus), and Dogs (Canis familiaris) from the United States [J] . Rosypal Alexa C, Hill Roderick, Lewis Samantha, Journal of Parasitology . 2011,第1期

机译：快速免疫色谱检测试纸的评估，用于检测实验感染来自负鼠（Didelphis virginiana），犰狳（Dasypus novemcinctus）和狗（Canis Friendlyis）的分离株实验感染的狗中的克氏锥虫抗体

4. Design of a rapid detection of hepatitis B Immuno gold dipstick test strip control system [C] . Yan Hou, Jinxia Huang International Conference on Machinery, Electronics and Control Simulation . 2014

机译：快速检测乙型肝炎免疫金代杆试验条控制系统的设计

5. A novel approach to control and regulate the classical complement pathway: Highly specific inhibition of C1q globular head binding to human IgG using an engineered single chain antibody variable fragment; recruiting C1q to HER2 /neu positive cells using truncated bispecific antibodies [D] . Hwang, Hee Young 2007

机译：一种控制和调节经典补体途径的新方法：使用工程化的单链抗体可变片段高度特异性地抑制C1q球头与人IgG的结合；使用截短的双特异性抗体将C1q募集到HER2 / neu阳性细胞

6. Single Chain Fragment Variable Recombinant Antibody Functionalized Gold Nanoparticles for a Highly Sensitive Colorimetric Immunoassay [O] . Yang Liu, Yi Liu, Raymond L. Raymond, -1

机译：单链片段可变重组抗体功能化的金纳米粒子的高灵敏度比色免疫测定。

7. Chemiluminescence immunoassay for the rapid and sensitive detection of antibody against porcine parvovirus by using horseradish peroxidase/detection antibody-coated gold nanoparticles as nanoprobes. Luminescence 2014; 29(4): 338-343. DOI 10.1002/bio.2747 [O] . Yuan Zhou, Tao Zhou, Rui Zhou, 2014

机译：用辣根过氧化物酶/检测抗体涂覆的金纳米颗粒作为纳米体，化学发光的免疫测定免疫分析，用于抗猪细小病毒的抗体抗体。发光2014; 29（4）：338-343。 DOI 10.1002 / BIO.2747

1. 从人源单链抗体库中筛出具有抗原结合活性的重链可变区和更小抗体片段 [J] . 胡志伟 ,段招军 ,朱建高 . 中国免疫学杂志 . 1999,第004期

2. 利用单克隆抗体技术及金标免疫分析法制备吗啡快速检测试纸 [J] . 陈实平 ,金淑敏 ,董红艳 . 中国医学科学院学报 . 1998,第006期

3. 免疫金标探针快速检测棘球蚴囊肿抗体 [J] . 张银霞 ,田红 ,于兰 . 西北国防医学杂志 . 2002,第002期

4. 基于抗单增李斯特菌单链抗体的胶体金探针制备及其活性鉴定 [J] . 刘爱平 ,叶子熊 ,马榆 . 食品科学 . 2017,第004期

5. 抗细粒棘球蚴人工重组B抗原人源单链可变区抗体的筛选、鉴定和免疫活性检测 [J] . 陈新华 ,温浩 ,张耀新 . 中华微生物学和免疫学杂志 . 2003,第001期

6. PRRSV GP5蛋白抗独特型抗体的单链可变区片段(SFv)转导MARC-145后抑制PRRSV感染的研究 [C] . 李琼毅 ,王向鹏 ,刘红亮 . 中国畜牧兽医学会畜牧兽医生物技术学分会暨中国免疫学会兽医免疫分会第十次学术研讨会 . 2014

7. 单域抗体m36.4和10E8单链可变区片段用于艾滋病基因治疗策略的研究 [A] . 唐笑然 . 2020

1. 特异结合CD3的抗体、抗原结合片段和单链抗体可变区片段及其应用 [P] . 中国专利： CN111662382A . 2020-09-15

2. 单链可变片段抗-CD133抗体及其用途 [P] . 中国专利： CN103068850A . 2013-04-24

3. isolated antigen binding region that is specific for cd38, isolated antibody or a functional fragment thereof that is specific for cd38, isolated antibody or a functional fragment thereof, isolated immunoglobulin, heavy chain variable of an isolated antigen binding region, variable light chain from an isolated antigen binding region, isolated nucleic acid sequence, nucleic acid sequence encoding a variable heavy chain from an isolated antigen binding region, nucleic acid sequence, vector, isolated cell, pharmaceutical composition, use of the pharmaceutical composition, method of inducing specific death of cd38-expressing tumor cells, method of detecting specific death of cd38-expressing tumor cells, method of detecting the presence of cd38 in a minipore source tissue or cell , cd38 detection method in a cd3-expressing erythrocyte 8 and diagnostic composition [P] . 外国专利： BRPI0618399A2 . 2011-08-30

机译：对cd38有特异性的分离的抗原结合区，对cd38有特异性的分离的抗体或其功能片段，分离的抗体或其功能片段，分离的免疫球蛋白，分离的抗原结合区的重链可变区，分离的可变轻链抗原结合区，分离的核酸序列，编码来自分离的抗原结合区的可变重链的核酸序列，核酸序列，载体，分离的细胞，药物组合物，药物组合物的用途，诱导cd38-特异性死亡的方法表达肿瘤细胞的方法，检测表达cd38的肿瘤细胞的特异性死亡的方法，检测小孔源组织或细胞中cd38的存在的方法，表达cd3的红细胞8的cd38检测方法和诊断组合物

4. pencicillin-binding protein, nucleic acid, antibody or antibody fragment, medicament, pharmaceutical composition, uses of at least one penicillin-binding protein or fragment or variant or fragment thereof, of at least one nucleic acid and of at least one antibody or antibody fragment, in vitro antibody detection methods directed against neisseria meningitidis infection from a penicillin-binding protein, nucleic acid, antibody or an antibody fragment, pharmaceutical composition of at least one penicillin-binding protein or fragment or variant or variant thereof, of at least one nucleic acid and at least one antibody directed against neisseria meningitidis infection from a biological sample of a mammal and in vitro diagnosis of neisseria miningitidis infection from a biological sample from a mammal and monoclonal antibody [P] . 外国专利： BRPI0610802A2 . 2016-11-16

机译：青霉素结合蛋白，核酸，抗体或抗体片段，药物，药物组合物，至少一种青霉素结合蛋白或其片段或变异体或片段，至少一种核酸和至少一种抗体或抗体片段的用途，针对脑膜炎奈瑟菌的青霉素结合蛋白，核酸，抗体或抗体片段感染的体外抗体检测方法，至少一种青霉素结合蛋白或其片段或变异体或变异体，至少一种核酸的药物组合物酸和至少一种针对哺乳动物生物样品中脑膜炎奈瑟氏菌感染的抗体以及来自哺乳动物生物样品中的矿业性奈瑟氏球菌感染的体外诊断和单克隆抗体

5. CHIMERIC MONOCLONAL ANTIBODY INTERACTING WITH HUMAN PLACENTAL ALKALINE PHOSPHATASE, VARIABLE REGION OF HEAVY -CHAIN OF CHIMERIC MONOCLONAL ANTIBODY, VARIABLE REGION OF LIGHT K-CHAIN OF CHIMERIC MONOCLONAL ANTIBODY, FRAGMENT OF CDNA ENCODING VARIABLE REGION OF HEAVY -CHAIN OF CHIMERIC MONOCLONAL ANTIBODY AND FRAGMENT OF CDNA ENCODING VARIABLE REGION OF LIGHT K-CHAIN OF CHIMERIC MONOCLONAL ANTIBODY [P] . 外国专利： RU2102479C1 . 1998-01-20

机译：嵌合单克隆抗体与人血浆碱性磷酸酶的相互作用，可变区重链的嵌合体，嵌合链型单克隆抗体轻度K链的可变区，嵌合DNA单核抗体的片段和CDNA的片段CDNA编码嵌合单克隆抗体轻K链的可变区

相关主题

A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick

摘要

著录项

相似文献

相关主题

期刊订阅