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首页> 外文期刊>RSC Advances >Folate-modified silicon carbide nanoparticles as multiphoton imaging nanoprobes for cancer-cell-specific labeling
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Folate-modified silicon carbide nanoparticles as multiphoton imaging nanoprobes for cancer-cell-specific labeling

机译：叶酸修饰的碳化硅纳米粒子作为多光子成像纳米探针，用于癌细胞特异性标记

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Interest in multiphoton microscopy for cell imaging has considerably increased over the last decade. Silicon carbide (SiC) nanoparticles exhibit strong second-harmonic generation (SHG) signal, and can thus be used as nonlinear optical probes for cell imaging. In this study, the surface of SiC nanoparticles was chemically modified to enable cancer-cell-specific labeling. In a first step, an aminosilane was grafted onto the surface of SiC nanoparticles. The resulting nanoparticles were further modified with folic acid, using an isothiocyanate-based coupling method. Nanoparticles from different functionalization steps were investigated by zeta potential measurement, colorimetric titration, infrared and ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray photoelectron spectroscopy (XPS), and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Characterization results confirmed successful covalent grafting of silane and folic acid to nanoparticle surface. Finally, the efficacy of these folate-modified SiC nanoparticles for cancer-cell-specific labeling was evaluated by multiphoton microscopy, by measuring SHG-emitting cell area on multiphoton images. The average cancer-cell labeling percentage was about 48%, significantly higher than for negative controls (healthy cells, competition assay and poly(ethylene glycol) modified-SiC nanoparticles), where it ranged between 10% and 15%. These results demonstrated good efficiency and specificity for these folate-modified SiC nanoparticles in cancer-cell-specific labeling.
机译：在过去的十年中，对用于细胞成像的多光子显微镜的兴趣大大增加。碳化硅（SiC）纳米颗粒表现出很强的二次谐波生成（SHG）信号，因此可以用作细胞成像的非线性光学探针。在这项研究中，对SiC纳米颗粒的表面进行了化学修饰，以实现癌细胞特异性标记。第一步，将氨基硅烷接枝到SiC纳米颗粒的表面上。使用基于异硫氰酸酯的偶联方法，将得到的纳米颗粒进一步用叶酸改性。通过zeta电位测量，比色滴定，红外和紫外可见（UV-Vis）吸收光谱，X射线光电子能谱（XPS）和飞行时间二次离子质谱（ToF-模拟人生）。表征结果证实了硅烷和叶酸成功共价接枝到纳米颗粒表面。最后，通过测量多光子图像上发射SHG的细胞面积，通过多光子显微镜评估了这些叶酸修饰的SiC纳米颗粒对癌细胞特异性标记的功效。平均癌细胞标记百分比约为48％，明显高于阴性对照（健康细胞，竞争测定法和聚（乙二醇）修饰的SiC纳米颗粒）的百分比，其范围在10％至15％之间。这些结果证明了这些叶酸修饰的SiC纳米颗粒在癌细胞特异性标记中具有良好的效率和特异性。

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