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Development of small-volume, microfluidic chaotic mixers for future application in two-dimensional liquid chromatography

机译:开发小体积,微流体混沌混合器,以备将来在二维液相色谱中的应用

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We report a microfluidic chaotic micromixer with staggered herringbone grooves having a geometry optimized for fast mobile-phase modification at the interface of a two-dimensional liquid chromatography system. The volume of the 300 μm mixers is 1.6 microliters and they provide mixing within 26 s at a flow rate of 4 μL min?1 and 0.09 s at a flow rate of 1000 μL min?1. Complete mixing is achieved within a distance of 3 cm along the 5 cm-long microchannel over the whole range of flow rates. The mixers can be used to mix aqueous phosphate-buffered saline solutions with methanol or acetonitrile at different ratios (1?:?2, 1?:?5 and 1?:?10). We also describe in detail an improved fabrication protocol for these mixers using a two-step soft photolithographic procedure. Mixers are made by replication in poly(dimethylsiloxane).
机译:我们报告的微流体混沌微混合器具有交错的人字形凹槽,具有在二维液相色谱系统的界面上针对快速流动相修饰而优化的几何形状。 300μm混合器的体积为1.6微升,它们在26 s内以4μLmin ?1 的流速提供混合,并在0.09 s的流速下提供混合。 μLmin ?1 。在整个流速范围内,沿着5厘米长的微通道在3厘米的距离内可以实现完全混合。混合器可用于将磷酸盐缓冲盐水溶液与甲醇或乙腈以不同的比例(1:2:,1:5和1:10)混合。我们还将详细介绍使用两步软光刻过程的这些混合器的改进的制造协议。混合器是在聚二甲基硅氧烷中复制制得的。

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