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首页> 外文期刊>RSC Advances >Identification of DNA single-base mismatches by resistivity of poly(N-isopropylacrylamide)-block-ssDNA copolymer brush films at dual temperatures
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Identification of DNA single-base mismatches by resistivity of poly(N-isopropylacrylamide)-block-ssDNA copolymer brush films at dual temperatures

机译：聚（Nem）N -异丙基丙烯酰胺）-嵌段 -ssDNA共聚物刷膜在双温度下的电阻率鉴定DNA单碱基错配

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We grafted azido-terminated poly(N-isopropylacrylamide) (PNIPAAm) brushes onto thin gold films on silicon as a bottom electrode. A probe of single-stranded DNA (ssDNA) presenting a 4-pentynoic acid succinimidyl ester unit was grafted onto the azido-terminated PNIPAAm brushes through a click reaction, resulting in block copolymer brushes. The PNIPAAm-b-ssDNA copolymer brushes formed homogeneous complexes stabilized via bio-multiple hydrogen bonds (BMHBs), which enhanced proton transfer and thereby decreased the resistivity of the structures. The homogeneous complex state of the PNIPAAm-b-ssDNA copolymer brushes transformed into a phase-separated state after hybridization with 0.5 pg nL?1 of its target DNA, which resulted from competition between the BMHBs and complementary HBs; this phase transformation of the PNIPAAm and probe segments inhibited proton transfer and significantly increased the resistivity. Furthermore, hybridization with mismatched DNA sequences generated sufficient “proton leakage” to decrease the resistivity at temperatures below the lower critical solution temperature (LCST), especially for adenine and guanine units. Sequences with thymine and cytosine mismatches could be distinguished from the target at temperatures above the LCST due to disruption of the BMHBs at increased temperatures (80 °C). The ability to detect label-free DNA and recognize sequence mismatches suggests the potential use of these novel materials in bioelectronics.
机译：我们将叠氮基封端的聚（ N -异丙基丙烯酰胺）（PNIPAAm）刷子接枝到硅上的金薄膜上作为底部电极。通过点击反应，将具有4-戊酸琥珀酰亚胺基酯单元的单链DNA（ssDNA）探针嫁接到叠氮基末端的PNIPAAm刷子上，得到嵌段共聚物刷子。 PNIPAAm- b -ssDNA共聚物刷形成了通过生物多氢键（BMHBs）稳定的稳定的均质复合物，从而增强了质子传递，从而降低了结构的电阻率。 PNIPAAm- b -ssDNA共聚物刷与0.5 pg nL ？1 杂交后均质复合状态转变为相分离状态其靶DNA是由BMHB和互补HB之间的竞争产生的; PNIPAAm和探针片段的这种相变抑制了质子转移并显着提高了电阻率。此外，与错配的DNA序列杂交产生足够的“质子泄漏”，以降低低于较低临界溶液温度（LCST）的温度下的电阻率，尤其是对于腺嘌呤和鸟嘌呤单元。胸腺嘧啶和胞嘧啶错配的序列可以在高于LCST的温度下与靶标区分开，这是因为在升高的温度（80°C）下BMHBs受到破坏。检测无标记DNA和识别序列错配的能力表明这些新材料在生物电子学中的潜在用途。

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