• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >ICCD camera technology with constant illumination source and possibilities for application in multiwavelength analytical ultracentrifugation
【24h】

ICCD camera technology with constant illumination source and possibilities for application in multiwavelength analytical ultracentrifugation

机译：具有恒定照明源的ICCD摄像头技术及其在多波长分析超速离心中的应用可能性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A new concept for multiwavelength detection in analytical ultracentrifugation is demonstrated, based on the technology of an Andor iStar intensified CCD camera and constant illumination source. The camera is coupled to an Andor Shamrock spectrograph providing a spectrometer system capable of UV/vis acquisitions with wavelengths from 190 to 790 nm. The details of the camera functions are described, and the essential operational modes demonstrated with proof of principle measurements. The Andor spectrometer system is proven to be much more sensitive than previous AUC detectors. In fact, it is so sensitive in high gain modes that signal quality is limited by photon shot-noise. Acquisition strategies are presented for detection systems working with low-light illumination sources, and the necessity of overcoming shot-noise limited signal quality is revealed. Several illumination optical concepts are tested and it is shown that when sufficient illumination is applied the detection system is capable of outperforming commercial and academic detectors previously reported in terms of signal-to-noise. A path forward for overcoming the remaining challenges is provided. The findings in this manuscript will be applicable to any type of high sensitivity detector design.
机译：基于安道尔iStar增强型CCD相机和恒定照明源的技术，展示了一种用于分析超速离心中多波长检测的新概念。摄像机与Andor Shamrock光谱仪耦合，提供了能够进行190/790 nm波长的UV / vis采集的光谱仪系统。描述了相机功能的细节，并通过原理测量证明了基本的操作模式。事实证明，安道尔光谱仪系统比以前的AUC检测器灵敏得多。实际上，它在高增益模式下是如此敏感，以至于信号质量受到光子散粒噪声的限制。提出了针对低照度光源的检测系统的采集策略，并揭示了克服散粒噪声限制的信号质量的必要性。测试了几种照明光学概念，结果表明，当施加足够的照明时，检测系统的性能将优于先前报告的信噪比的商业和学术检测器。提供了克服剩余挑战的前进之路。本手稿中的发现适用于任何类型的高灵敏度检测器设计。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号