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Decorating a single giant DNA with gold nanoparticles

机译：用金纳米粒子装饰单个巨型DNA

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We decorated a single giant DNA (1.66 × 10 ~(5) base pairs) with gold nanoparticles through the simple procedure of mild warming, without denaturation of the DNA molecule. Single-molecule observation with fluorescence microscopy revealed that individual decorated DNA molecules stay in the bulk solution by avoiding aggregation and precipitation, and exhibit translational and conformational fluctuation, i.e. , Brownian motion. An analysis of the intra-chain fluctuation of single DNA molecules revealed that the apparent spring constant and damping coefficient of a DNA chain increased by ca. 13- and 5-fold, respectively, upon decoration with gold nanoparticles. Observation by transmission electron microscopy revealed that gold nanoparticles were stably attached to the DNA skeleton. UV-visible measurements revealed the absence of any detectable change in surface plasmon resonance, suggesting that the gold nanoparticles assemble without the formation of a densely packed aggregate. CD measurements showed that the secondary structure of decorated DNA is still essentially the B-form.
机译：我们通过温和升温的简单程序用金纳米颗粒修饰了一个巨大的DNA（1.66×10〜（5）个碱基对），而不会使DNA分子变性。用荧光显微镜的单分子观察表明，通过避免聚集和沉淀，单个修饰的DNA分子保留在本体溶液中，并表现出翻译和构象波动，即布朗运动。对单个DNA分子的链内波动的分析表明，一条DNA链的表观弹簧常数和阻尼系数大约增加了。用金纳米颗粒装饰时分别为13倍和5倍。通过透射电子显微镜观察发现，金纳米颗粒稳定地附着在DNA骨架上。紫外可见测量表明，表面等离振子共振没有任何可检测到的变化，这表明金纳米颗粒组装而没有形成密集堆积的聚集体。 CD测量表明装饰的DNA的二级结构仍然基本上是B-形式。

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