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首页> 外文期刊>RSC Advances >Guanine deaminase provides evidence of the increased caffeine content during the piling process of pu'erh tea
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Guanine deaminase provides evidence of the increased caffeine content during the piling process of pu'erh tea

机译：鸟嘌呤脱氨酶提供了普'茶打桩过程中咖啡因含量增加的证据

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Wet piling is a key process for producing pu'erh tea because various components change under the action of microorganisms. Among these components, caffeine content is increased. Evidence has indicated a salvage pathway for caffeine biosynthesis in microbes, in which xanthine is methylated in the order of N-3 → N-1 → N-7. In addition, guanine can be used to synthesize xanthine through guanine deaminase (EC: 3.5.4.3). In this study, we investigated the variation in caffeine content during piling fermentation with supplementary guanine, ~(15) N-labeled guanine and xanthine. We cloned the guanine deaminase gene ( GUD1 ) from Saccharomyces cerevisiae (one dominant strain in piling fermentation). The results revealed that [ ~(15) N]xanthine could be synthesized from [ ~(15) N]guanine, and [ ~(15) N]caffeine was also detected during piling with supplementary [ ~(15) N]xanthine. Furthermore, ScGUD1 could catalyze the conversion of guanine to xanthine, which is likely to be methylated for caffeine synthesis under microorganism action. The obtained results revealed the mechanism underlying the increased caffeine content during piling of pu'erh tea.
机译：湿法堆积是生产普'茶的关键过程，因为各种成分会在微生物的作用下发生变化。在这些成分中，咖啡因含量增加。证据表明微生物中咖啡因生物合成的挽救途径，其中黄嘌呤以N-3→N-1→N-7的顺序被甲基化。另外，鸟嘌呤可用于通过鸟嘌呤脱氨酶（EC：3.5.4.3）来合成黄嘌呤。在这项研究中，我们调查了补充鸟嘌呤，〜（15）N标记鸟嘌呤和黄嘌呤堆发酵过程中咖啡因含量的变化。我们从酿酒酵母（堆发酵中的一个主要菌株）克隆了鸟嘌呤脱氨酶基因（GUD1）。结果表明，可以从[〜（15）N]鸟嘌呤合成[〜（15）N]黄嘌呤，在补充[〜（15）N]黄嘌呤的过程中也检测到[〜（15）N]咖啡因。此外，ScGUD1可以催化鸟嘌呤向黄嘌呤的转化，黄嘌呤在微生物作用下可能被甲基化以合成咖啡因。获得的结果揭示了普iling茶堆放过程中咖啡因含量增加的潜在机理。

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