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首页> 外文期刊>RSC Advances >Biofunctionalization of decellularized porcine aortic valve with OPG-loaded PCL nanoparticles for anti-calcification
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Biofunctionalization of decellularized porcine aortic valve with OPG-loaded PCL nanoparticles for anti-calcification

机译：OPG负载PCL纳米粒子对脱细胞猪主动脉瓣的生物功能化以抗钙化

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Decellularized valve stents are widely used in tissue-engineered heart valves because they maintain the morphological structure of natural valves, have good histocompatibility and low immunogenicity. However, the surface of the cell valve loses the original endothelial cell coverage, exposing collagen and causing calcification and decay of the valve in advance. In this study, poly ε-caprolactone (PCL) nanoparticles loaded with osteoprotegerin (OPG) were bridged to a decellularized valve using a nanoparticle drug delivery system and tissue engineering technology to construct a new anti-calcification composite valve with sustained release function. The PCL nanoparticles loaded with OPG were prepared via an emulsion solvent evaporation method, which had a particle size of 133 nm and zeta potential of ?27.8 mV. Transmission electron microscopy demonstrated that the prepared nanoparticles were round in shape, regular in size, and uniformly distributed, with an encapsulation efficiency of 75%, slow release in vitro , no burst release, no cytotoxicity to BMSCs, and contained OPG nanoparticles in vitro . There was a delay in the differentiation of BMSCs into osteoblasts. The decellularized valve modified by nanoparticles remained intact and its collagen fibers were continuous. After 8 weeks of subcutaneous implantation in rats, the morphological structure of the valve was almost complete, and the composite valve showed anti-calcification ability to a certain extent.
机译：脱细胞瓣膜支架因其保持天然瓣膜的形态结构，良好的组织相容性和低免疫原性而被广泛用于组织工程心脏瓣膜。然而，细胞瓣膜的表面失去了最初的内皮细胞覆盖，暴露了胶原蛋白并导致了瓣膜的钙化和腐烂。在这项研究中，使用纳米颗粒药物递送系统和组织工程技术将负载有骨保护素（OPG）的聚ε-己内酯（PCL）纳米颗粒桥接至脱细胞瓣膜，以构建具有缓释功能的新型抗钙化复合瓣膜。通过乳液溶剂蒸发法制备了负载有OPG的PCL纳米颗粒，其粒径为133nm，ζ电势为〜27.8mV。透射电子显微镜显示所制备的纳米颗粒为圆形，尺寸规则且均匀分布，包封率为75％，体外缓慢释放，没有爆发释放，对BMSCs无细胞毒性，并且在体外含有OPG纳米颗粒。 BMSCs向成骨细胞的分化有所延迟。纳米粒子修饰的脱细胞瓣膜保持完整，其胶原纤维是连续的。大鼠皮下植入8周后，瓣膜的形态结构几乎完整，复合瓣膜在一定程度上显示出抗钙化能力。

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