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Development of a flow-free magnetic actuation platform for an automated microfluidic ELISA

机译：开发用于自动微流ELISA的无流量磁驱动平台

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There is a need to create an easily deployable and point-of-care (POC) diagnostic platform for disease outbreaks and for monitoring and maintenance of chronic illnesses. Such platforms are useful in regions where access to clinical laboratories may be limited or constrained using cost-effective solutions to quickly process high numbers of samples. Using oil and water liquid–liquid interphase separation, immunoassays developed for microfluidic chips can potentially meet this need when leveraged with electromagnetic actuation and antibody-coated superparamagnetic beads. We have developed a microfluidic immunoassay detection platform, which enables assay automation and maintains successful liquid containment for future use in the field. The assay was studied through a series of magnetic and fluid simulations to demonstrate these optimizations, and an optimized chip was tested using a target model for HIV-1, the p24 capsid antigen. The use of minimal reagents further lowers the cost of each assay and lowers the required sample volume for testing (<50 μL), that can offer easy turnaround for sample collection and assay results. The developed microfluidic immunoassay platform can be easily scaled for multiplex or multi-panel specific testing at the POC.
机译：需要创建一个易于部署的即时医疗点（POC）诊断平台，用于疾病暴发以及监测和维护慢性病。此类平台在使用成本有效的解决方案来快速处理大量样本的临床实验室的访问可能受到限制或限制的地区很有用。利用油和水的液-液相分离，为微流控芯片开发的免疫分析法在与电磁激励和抗体包被的超顺磁珠结合使用时，有可能满足这一需求。我们已经开发了一种微流体免疫分析检测平台，该平台可实现分析自动化并保持成功的液体容纳，以备将来在该领域中使用。通过一系列磁和流体模拟研究了该检测方法，以证明这些优化方法，并使用针对HIV-1（p24衣壳抗原）的靶模型测试了优化的芯片。最少试剂的使用进一步降低了每次测定的成本，并降低了测试所需的样品量（<50μL），可轻松实现样品收集和测定结果的周转。开发的微流体免疫分析平台可以轻松扩展到POC的多重或多面板特异性测试。

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