Acrylamide-dT: a polymerisable nucleoside for DNA incorporation

Francia Allabush; Paula M. Mendes; James H. R. Tucker

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Acrylamide-dT: a polymerisable nucleoside for DNA incorporation

机译：丙烯酰胺-dT：DNA掺入的可聚合核苷

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The synthesis of a novel modified nucleoside phosphoramidite, Acrylamide-dT-CE phosphoramidite, obtained in three steps from commercially available starting materials, is reported. It was readily incorporated into thrombin binding aptamer ( TBA ) sequences using automated solid-phase synthesis under ultra-mild conditions, with the modification shown not to adversely affect duplex stability, G-quadruplex structure, or thrombin binding. The reaction and integration of the modified strands with acrylamide polymers was evidenced by gel electrophoresis. The Acrylamide-dT functional handle promises to be an ideal synthon for preparing DNA–polymer hybrids for use in various macromolecular materials applications.

机译：据报道，从市售起始原料中分三步合成了一种新型的改性核苷亚磷酰胺，丙烯酰胺-dT-CE亚磷酰胺。在超温和的条件下使用自动化固相合成法，可以很容易地将其掺入凝血酶结合适体（TBA）序列中，修饰显示不会对双链体稳定性，G-四链体结构或凝血酶结合产生不利影响。凝胶电泳证明了改性链与丙烯酰胺聚合物的反应和整合。丙烯酰胺-dT功能手柄有望成为制备用于各种高分子材料的DNA-聚合物杂化物的理想合成子。

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《RSC Advances》 |2019年第54期|共6页
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Francia Allabush; Paula M. Mendes; James H. R. Tucker;
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1. Acrylamide-dT: a polymerisable nucleoside for DNA incorporation [J] . RSC Advances . 2019,第54期

机译：丙烯酰胺-DT：用于DNA掺入的可聚合核苷酸
2. Alkylsulfanylphenyl Derivatives of Cytosine and 7-Deazaadenine Nucleosides, Nucleotides and Nucleoside Triphosphates: Synthesis, Polymerase Incorporation to DNA and Electrochemical Study [J] . Dr. Hana Macíčková-Cahová, Dr. Radek Pohl, Dr. Petra Horáková, Chemistry - A European Journal . 2011,第21期

机译：胞嘧啶和7-脱氮杂腺嘌呤核苷，核苷酸和核苷三磷酸的烷基硫烷基苯基衍生物：合成，聚合酶掺入DNA和电化学研究
3. Alkylsulfanylphenyl derivatives of cytosine and 7-deazaadenine nucleosides, nucleotides and nucleoside triphosphates: Synthesis, polymerase incorporation to DNA and electrochemical study [J] . Mací?ková-Cahová H., Pohl R., Horáková P., Chemistry: A European journal . 2011,第21期

机译：胞嘧啶和7-脱氮杂腺嘌呤核苷，核苷酸和三磷酸核苷的烷基硫烷基苯基衍生物：合成，聚合酶掺入DNA和电化学研究
4. Synthesis of fluorescent nucleoside analogues and their applicationas dual probes for the non-canonical DNA structures [C] . Wen Ann Wee, Jihye Yum, ShingoHirashima 日本化学会春季年会 . 2020

机译：非规范DNA结构的荧光核苷类似物的合成及其适用性双探针
5. Enzyme-Mediated Site-Specific Incorporation of a Fluorescent Nucleoside into RNA: Method and Applications [D] . Li, Yao 2016

机译：酶介导的将特定位置的荧光核苷掺入RNA：方法和应用
6. Expanding the repertoire of DNA polymerase substrates: template-instructed incorporation of non-nucleoside triphosphate analogues by DNA polymerases β and λ [O] . Emmanuele Crespan, Ludmila Alexandrova, Anastasiya Khandazhinskaya, 2007

机译：扩展DNA聚合酶底物库：DNA聚合酶β和λ指导模板指导的非核苷三磷酸类似物掺入
7. Acrylamide-dT: a polymerisable nucleoside for DNA incorporation [O] . Francia Allabush, Paula M. Mendes, James H. R. Tucker 2019

机译：丙烯酰胺-DT：用于DNA掺入的可聚合核苷酸

Acrylamide-dT: a polymerisable nucleoside for DNA incorporation

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