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首页> 外文期刊>RSC Advances >Untargeted metabolomics using liquid chromatography coupled with mass spectrometry for rapid discovery of metabolite biomarkers to reveal therapeutic effects of Psoralea corylifolia seeds against osteoporosis
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Untargeted metabolomics using liquid chromatography coupled with mass spectrometry for rapid discovery of metabolite biomarkers to reveal therapeutic effects of Psoralea corylifolia seeds against osteoporosis

机译：使用液相色谱和质谱联用的非靶向代谢组学，可快速发现代谢物生物标志物，以揭示补骨脂种子对骨质疏松症的治疗作用

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Liquid chromatography coupled with mass spectrometry has been used as metabolomics profiling tool to discover and identify the metabolites in metabolic diseases. Osteoporosis (OP) syndrome is a chronic metabolic disease characterized by bone mass reduction and changes in bone microstructure. Psoralea corylifolia Linn. seeds (PCS) have a therapeutic effect on osteoporosis, but their action mechanism and therapeutic target are still unclear. This study aims to explore the metabolic changes of the urine profile in glucocorticoid-induced OP model rats and the therapeutic effect of PCS. High-throughput metabolomics based on the liquid chromatography coupled with mass spectrometry quadrupole time-of-flight mass spectrometry and multivariate data analysis were used to analyze the urine metabolites. The results showed that has an obvious separation between model and control groups. OPLS-DA was used to further analyze and discover substances that contributed to the separation. 42 potential biomarkers and 12 related metabolic pathways were identified in combination with network databases. After the intervention of PCS, 24 biomarkers were significantly regulated, mainly with glycone, serine and threonine metabolism, glutathione metabolism and purine metabolism and other metabolic pathways are related and discovered. This study has proved that PCS has therapeutic effect against OP by regulating that metabolic pathways disturbed in the OP. It provided a basis for the research and future development of new drugs for OP treatment.
机译：液相色谱与质谱联用已被用作代谢组学分析工具，以发现和鉴定代谢性疾病中的代谢物。骨质疏松症（OP）综合征是一种慢性代谢性疾病，其特征是骨量减少和骨微结构改变。补骨脂。种子（PCS）对骨质疏松症有治疗作用，但其作用机理和治疗目标仍不清楚。本研究旨在探讨糖皮质激素诱导的OP模型大鼠尿液代谢的改变以及PCS的治疗作用。基于液相色谱的高通量代谢组学结合质谱四极杆飞行时间质谱和多元数据分析来分析尿液代谢物。结果表明，模型组和对照组之间有明显的分离。 OPLS-DA用于进一步分析和发现有助于分离的物质。结合网络数据库，鉴定出42种潜在的生物标志物和12种相关的代谢途径。 PCS干预后，有24种生物标记被显着调节，主要与甘氨酸，丝氨酸和苏氨酸代谢，谷胱甘肽代谢和嘌呤代谢等代谢途径相关并被发现。这项研究证明，PCS通过调节OP中的代谢途径而对OP具有治疗作用。它为OP治疗新药的研究和未来开发提供了基础。

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