• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >The effect of healing phenotype-inducing cytokine formulations within soft hydrogels on encapsulated monocytes and incoming immune cells
【24h】

The effect of healing phenotype-inducing cytokine formulations within soft hydrogels on encapsulated monocytes and incoming immune cells

机译：软水凝胶中愈合表型诱导细胞因子制剂对包封的单核细胞和传入免疫细胞的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The adverse immune responses to implantable biomedical devices is a general problem with important consequences for the functionality of implants. Immunomodulatory soft hydrogel-based interfaces between the implant and the host can attenuate these reactions. Moreover, encapsulation of the patient's own immune cells into these interfaces can lead to the personalisation of implants from the immune reaction point of view. Herein, we described a co-crosslinkable composite hydrogel (composed of gelatin and hyaluronic acid), which could be used for the encapsulation of macrophages in the presence of an anti-inflammatory phenotype-fixing cytokine cocktail. To mimick the incoming immune cells on the coating surface in vivo , peripheral blood mononuclear cells were seeded on the hydrogels. The encapsulation of monocytic cells into the composite hydrogels in the presence of cytokine cocktails at 5× or 10× concentrations led to the spreading of the encapsulated cells instead of the formation of clusters. Moreover, the secretion of the anti-inflammatory cytokines IL-1RA and CCL-18 was significantly increased. The attachment of PBMC to the surface of the hydrogel is dependent on the hydrogel composition and also significantly increased in the presence of the cytokine cocktail together with the number of CD68+ cells on the hydrogel surface. Our study demonstrates that the delivery of a polarisation cocktail with biocompatible hydrogels can control the initial response by the incoming immune cells. This effect can be improved by the encapsulation of autologous monocytes that are also polarised by the cytokine cocktail and secrete additional anti-inflammatory cytokines. This interface can fine tune the initial immune response to an implanted biomaterial in a personalised manner.
机译：对植入式生物医学装置的不良免疫反应是一个普遍的问题，对植入物的功能具有重要的影响。植入物和宿主之间基于免疫调节软水凝胶的界面可以减弱这些反应。此外，从免疫反应的观点来看，将患者自身的免疫细胞封装到这些界面中可以导致植入物的个性化。在本文中，我们描述了一种可共交联的复合水凝胶（由明胶和透明质酸组成），可在存在抗炎表型的细胞因子鸡尾酒的情况下用于巨噬细胞的封装。为了模拟体内涂层表面上传入的免疫细胞，将外周血单核细胞接种在水凝胶上。在浓度为5x或10x的细胞因子混合物的情况下，单核细胞被包囊到复合水凝胶中，导致被包囊的细胞扩散而不是形成簇。而且，抗炎细胞因子IL-1RA和CCL-18的分泌显着增加。 PBMC在水凝胶表面的附着取决于水凝胶的组成，并且在细胞因子混合物的存在下以及水凝胶表面CD68 +细胞的数量也显着增加。我们的研究表明，带有生物相容性水凝胶的极化混合物的递送可以控制传入免疫细胞的初始反应。通过封装也被细胞因子混合物极化并分泌其他抗炎细胞因子的自体单核细胞的封装，可以改善这种效果。该界面可以个性化方式微调对植入生物材料的初始免疫反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号