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Immobilization adjusted clock reaction in the urea–urease–H+ reaction system

机译：在尿素-尿素酶-H +反应系统中固定化调节时钟反应

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The bell-shaped reactivity-pH curve is the fundamental reason that the temporal programmable kinetic switch in clock reactions can be obtained in bio-competitive enzymatic reactions. In this work, urease was loaded on small resin particles through ionic binding. Experimental results reveal that the immobilization not only increased the stability of the enzyme and the reproducibility of the clock reaction, but also shifted the bell-shaped activity curve to lower pHs. The latter change enables the clock reaction to occur from an initial pH of 2.3, where the free enzyme had already lost its activity. Two mechanisms explain the influence of the immobilization on the clock reaction. Immobilization modified the pH sensitive functional groups on the enzyme, shifting the activity curve to a more acidic region, and reduced diffusion alters the enzyme dynamics.
机译：钟形反应性-pH曲线是在生物竞争性酶促反应中可以获得时钟反应中的时间可编程动力学转换的根本原因。在这项工作中，脲酶通过离子键合负载在小的树脂颗粒上。实验结果表明，固定化不仅增加了酶的稳定性和时钟反应的可重复性，而且使钟形活性曲线移至较低的pH值。后一种变化使时钟反应从初始pH值2.3开始发生，此时游离酶已经失去活性。两种机制解释了固定化对时钟反应的影响。固定化修饰了酶上的pH敏感官能团，将活性曲线移至更酸性的区域，减少的扩散改变了酶的动力学。

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