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The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material

机译：多孔二氧化硅材料固定的柚皮苷酶的特性和水解性能的研究

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Silica material has high specific surface area and excellent chemical stability, which make it useful for enzyme immobilization. In this work, naringinase was immobilized from fermentation broth of Aspergillus niger FFCC uv-11 by silica materials with different pore diameters of 2?nm (MCM-41), 7.7 nm (SBA-15) and 80 nm (silica gel). It was shown that SBA-15 had the highest naringinase activity, and this was chosen as a suitable carrier material for naringinase immobilization. First, SBA-15 was modified by glutaraldehyde at a concentration of 7% at 25 °C for 2 h, and it was then used for the immobilization of naringinase. At pH 3.5, the immobilized naringinase activity reached 467.62 U g ~(?1) at 40 °C for 4 h when the initial naringinase activity was 89.04 U mL ~(?1) . Furthermore, at the optimal reaction temperature of 45 °C and pH of 4.5, the binding efficiency, activity recovery rate and specific activity of the immobilized naringinase were 63.66%, 87.64% and 517.43 U g ~(?1) , respectively. Compared with free naringinase, in naringin hydrolysis, the immobilized naringinase performed over a wide pH application range and had good thermal stability. Even more important, the immobilized naringinase retained 61.81% of the residual naringinase activity after eight consecutive cycles, and kept 80.95% of the residual naringinase activity after one month of storage. This study provides an ideal carrier material and some basic data for naringinase immobilization technology, which will greatly promote the application of naringinase in industrial fruit juice processing.
机译：二氧化硅材料具有高的比表面积和优异的化学稳定性，这使其可用于酶固定化。在这项工作中，通过不同孔径分别为2？nm（MCM-41），7.7 nm（SBA-15）和80 nm（硅胶）的二氧化硅材料，从黑曲霉FFCC uv-11的发酵液中固定了柚皮苷酶。结果表明，SBA-15具有最高的柚皮苷酶活性，因此被选作适合于柚皮苷酶固定的载体材料。首先，将戊二醛在25°C下以7％的浓度戊二醛修饰SBA-15 2小时，然后将其用于柚皮苷酶的固定化。在pH 3.5下，当初始柚皮苷酶活性为89.04 U mL〜（？1）时，在40°C下固定的柚皮苷酶活性在4°C下达到467.62 U g〜（？1）。此外，在最适反应温度为45℃和pH为4.5时，固定的柚皮苷酶的结合效率，活性恢复率和比活性分别为63.66％，87.64％和517.43 U g〜（？1）。与游离的柚皮苷酶相比，在柚皮苷水解中，固定的柚皮苷酶在较宽的pH应用范围内均具有良好的热稳定性。更为重要的是，固定的柚皮苷酶在连续八个循环后保留了61.81％的柚皮苷酶残留活性，并在储存一个月后保留了80.95％的柚皮苷酶残留活性。该研究为柚皮苷酶固定化技术提供了理想的载体材料和一些基础数据，将大大促进柚皮苷酶在工业果汁加工中的应用。

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