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Fluorometric enhancement of the detection of H2O2 using different organic substrates and a peroxidase-mimicking polyoxometalate

机译：荧光增强使用不同的有机底物和过氧化物酶模拟多金属氧酸盐检测H2O2的能力

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摘要

Simple, sensitive and stable fluorometric sensors based on the polyoxotungstate intrinsic peroxidase (Na _(10) [α-SiW _(9) O _(34) ]) induced fluorescent enhancement of benzoic acid (BA), thiamine (TH) and 3-(4-hydroxyphenyl)propionic acid (HPPA) for the detection of hydrogen peroxide (H _(2) O _(2) ) are developed for the first time. In three assays, the three non-fluorescent substrates BA, TH and HPPA were oxidized with the ·OH radicals decomposed from H _(2) O _(2) under the catalysis of Na _(10) [α-SiW _(9) O _(34) ] under basic pH conditions. The optimal conditions for the detection of H _(2) O _(2) were evaluated and possible mechanisms are also discussed. The fluorescence intensity increases were linearly related to the concentration of H _(2) O _(2) in the ranges 1 × 10 ~(?8) to 1.6 × 10 ~(?6) , 1.6 × 10 ~(?6) to 1 × 10 ~(?4) , and 1 × 10 ~(?5) to 2.5 × 10 ~(?4) M with BA, TH, and HPPA as substrates, respectively. Detection limits for the three systems were found to be 6.7 × 10 ~(?9) , 2.2 × 10 ~(?7) and 9.6 × 10 ~(?6) M (3 σ ), respectively. The RSD values ranged from 2.57% to 4.66%, 0.82% to 4.06%, and 1.08% to 2.75%, respectively. The rates of recoveries were between 99.73% and 113.06%, 95.20% and 104.22%, and 95.28% and 128.76%, respectively. Moreover, the effects of interference were studied. The proposed work was successfully applied to the determination of H _(2) O _(2) in water and a sensitive, rapid and easy to operate assay was built, which has great potential applications in environmental science.
机译：简单，敏感和稳定的荧光传感器基于聚氧钨酸固有过氧化物酶（Na _（10）[α-SiW_（9）O _（34）]）诱导苯甲酸（BA），硫胺素（TH）和3的荧光增强首次开发了用于检测过氧化氢（H _（2）O _（2））的-（4-羟苯基）丙酸（HPPA）。在三种测定中，三种非荧光底物BA，TH和HPPA在Na _（10）[α-SiW_（9）的催化下被从H _（2）O _（2）分解的·OH自由基氧化。 ）O _（34）]在碱性pH条件下。评估了检测H _（2）O _（2）的最佳条件，并讨论了可能的机制。荧光强度的增加与H _（2）O _（2）的浓度在1×10〜（？8）至1.6×10〜（？6），1.6×10〜（？6）的范围内呈线性关系。分别以BA，TH和HPPA为底物的1×10〜（？4）和1×10〜（？5）至2.5×10〜（？4）M。发现这三个系统的检出限分别为6.7×10〜（？9），2.2×10〜（？7）和9.6×10〜（？6）M（3σ）。 RSD值分别为2.57％至4.66％，0.82％至4.06％和1.08％至2.75％。回收率分别在99.73％和113.06％之间，95.20％和104.22％之间以及95.28％和128.76％之间。此外，还研究了干扰的影响。拟议的工作成功地用于水中H_（2）O_（2）的测定，并建立了灵敏，快速且易于操作的测定方法，在环境科学中具有巨大的潜在应用。

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