A clean and effective potassium hydroxide pretreatment of corncob residue for the enhancement of enzymatic hydrolysis at high solids loading

Xuewen Chi; Chao Liu; Yan-Hong Bi; Guang Yu; Yuedong Zhang; Zhaoyu Wang; Bin Li; Qiu Cui

摘要

Corncob residue (CCR) is an economic feedstock with enormous potential for the production of bioethanol. In this work, potassium hydroxide (KOH) pretreatment of CCR was investigated under relatively mild conditions, and the effectiveness of KOH pretreatment was evaluated by enzymatic saccharification at high solid loading. Results showed that after KOH pretreatment (16 wt% KOH dosage at 70 °C for 90 min) and the enzymatic hydrolysis at 20% solids loading and 20 FPU g ~(?1) -substrate of cellulase loading, the glucose yield could reach up to about 91%, which was over 90% higher compared to the raw CCR without KOH pretreatment. Correspondingly, about 89% of lignin and 79% of extractives were removed after KOH pretreatment. In addition, the spent liquor of KOH pretreatment containing sylvite could be used as lignin-based fertilizer based on the concept of biorefinery. In this case, the entire process for the production of fermentable sugars was clean and sustainable, which is very vital for the conversion of lignocelluloses to bioenergy or chemicals.

机译：玉米芯残留物（CCR）是一种经济原料，具有生产生物乙醇的巨大潜力。在这项工作中，在相对温和的条件下研究了CCR的氢氧化钾（KOH）预处理，并通过高固体量的酶促糖化作用评估了KOH预处理的有效性。结果表明，在KOH预处理（70％的COH剂量为16 wt％KOH，持续90分钟）并在20％固体负载和20 FPU g〜（？1）-纤维素酶负载底物的条件下进行酶水解，葡萄糖产量可以达到达到约91％，比未经KOH预处理的原始CCR高出90％以上。相应地，在KOH预处理之后，除去了约89％的木质素和79％的提取物。此外，基于生物精炼的概念，含有钾盐的KOH预处理废液可以用作木质素基肥料。在这种情况下，生产可发酵糖的整个过程是清洁且可持续的，这对于将木质纤维素转化为生物能或化学物质至关重要。

A clean and effective potassium hydroxide pretreatment of corncob residue for the enhancement of enzymatic hydrolysis at high solids loading

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