Objective . The activity of lysosomal enzymes is increased in the cerebrospinal fluid of bacterial meningitis, suggesting that inflammation may cause leakage of lysosomal enzymes into the extracellular fluid. Our objective was to study the activity of 3 lysosomal enzymes in cell-free peritoneal fluid of patients with peritoneal inflammation.Methods . The β-galactosidase, β-glucuronidase, and α-mannosidase activity (nmol 4-methylumbelliferone/mL/h); the total, polymorphonuclear, and mononuclear cell number; and chemical parameters were determined in the peritoneal fluid of 26 patients with culture-positive acute bacterial peritonitis, 13 patients (under antibiotic treatment) with culture-negative bacterial peritonitis, 6 patients with acute mesenteric lymphadenitis, and 26 control subjects who were operated on for surgical conditions without peritoneal inflammation.Results . The median β-galactosidase activity in the culture-positive bacterial peritonitis, mesenteric lymphadenitis, and controls was 175 (range: 63–2210), 50 (range: 37–56), and 16 (range: 8–32), respectively. The β-glucuronidase was 488 (range: 79–998), 53 (range: 27–98), and 15 (range: 3–22), respectively. The α-mannosidase was 801 (range: 100-3172), 78 (range: 33–157), and 41 (range: 16–63), respectively. The differences of the enzyme activities among the groups of the subjects studied were significant, with the exception of the α-mannosidase activity between mesenteric lymphadenitis and controls. There was no significant correlation between the enzyme activities and the cytologic or chemical parameters studied.Conclusions . The elevation of the lysosomal enzymes’ activity in the peritoneal fluid of patients with bacterial peritonitis seems to be a reliable index of peritoneal infection. Of the enzymes studied, the β-glucuronidase and β-galactosidase activities provide the best means for diagnosing bacterial inflammation of the peritoneal cavity.
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机译：目标。细菌性脑膜炎的脑脊液中溶酶体酶的活性增加，提示炎症可能导致溶酶体酶渗入细胞外液。我们的目的是研究腹膜炎患者无细胞腹膜液中的三种溶酶体酶的活性。 β-半乳糖苷酶，β-葡萄糖醛酸苷酶和α-甘露糖苷酶活性（nmol 4-甲基伞形酮/ mL / h）;总，多形核和单核细胞数;在26例培养阳性的急性细菌性腹膜炎患者，13例（抗生素治疗）培养阴性的细菌性腹膜炎患者，6例急性肠系膜淋巴结炎患者和26例接受手术的对照者的腹腔液中测定了腹膜液和化学参数。没有腹膜炎症的手术条件。在培养阳性细菌性腹膜炎，肠系膜淋巴结炎和对照中，β-半乳糖苷酶的中位活性分别为175（范围：63–2210），50（范围：37–56）和16（范围：8–32）。 β-葡萄糖醛酸苷酶分别为488（范围：79–998），53（范围：27–98）和15（范围：3–22）。 α-甘露糖苷酶分别为801（范围：100-3172），78（范围：33-157）和41（范围：16-63）。除肠系膜淋巴结炎和对照组之间的α-甘露糖苷酶活性外，研究对象组之间的酶活性差异显着。酶活性与所研究的细胞学或化学参数之间无显着相关性。结论。细菌性腹膜炎患者腹膜液中溶酶体酶活性的升高似乎是腹膜感染的可靠指标。在所研究的酶中，β-葡萄糖醛酸苷酶和β-半乳糖苷酶活性为诊断腹膜腔细菌炎症提供了最佳方法。
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