Grape Seed Extract Given Three Hours After Injury Suppresses Lipid Peroxidation and Reduces Hypoxic-Ischemic Brain Injury in Neonatal Rats

Yangzheng Feng; Yi-Ming Liu; Michael H Leblanc; Abhay J Bhatt; Philip G Rhodes

摘要

We have reported that pretreatment with grape seed extract (GSE), a potent antioxidant, is neuroprotective. This study examined whether treatment after injury with GSE is protective. Seven-day-old rat pups had the right carotid artery ligated, and then 2.5 h of 8% oxygen. GSE (50 mg/kg) or vehicle was administered by i.p. initial injection at 5 min to 5 h after reoxygenation, with an additional three doses within 26 h after injury. Brain damage was evaluated by weight deficit of the right hemisphere at 22 d after hypoxia. Treatment at 3 h after reoxygenation reduced brain weight loss from 21.0 ± 3.3% in vehicle-treated pups (n = 31) to 11.4 ± 2.8% in treated pups (n = 31, p 2α (8-isoPGF2α) and reduced an HI-induced increase in the proapoptotic protein c-jun in the brain cortex. GSE up to 3 h after reoxygenation reduces brain injury in rat pups, probably by suppressing lipid peroxidation and the proapoptotic protein c-jun.Abbreviations: GSE, grape seed extract; HI, hypoxia-ischemia, hypoxic-ischemic; 8-isoPGF2α, 8-isoprostaglandin F2α

机译：我们已经报道，用葡萄籽提取物（GSE）（一种有效的抗氧化剂）进行预处理具有神经保护作用。这项研究检查了GSE损伤后的治疗是否具有保护性。七日龄的幼犬结扎了右颈动脉，然后结扎了2.5小时的8％氧气。 GSE（50 mg / kg）或赋形剂通过腹膜内给药。复氧后5分钟至5小时首次注射，受伤后26小时内再注射3剂。通过缺氧后22 d右半球的体重不足来评估脑损伤。复氧后3小时的治疗将脑重量减轻从接受媒介物处理的幼犬（n = 31）的21.0±3.3 ％降低至接受治疗的幼犬（n = 31，p2α（8-isoPGF2α）的11.4±2.8 ％ HI诱导的大脑皮层中促凋亡蛋白c-jun的增加;再氧化后长达3小时的GSE可减轻大鼠幼鼠的脑损伤，可能是通过抑制脂质过氧化和促凋亡蛋白c-jun来实现的。 ; HI，缺氧缺血，缺氧缺血;8-isoPGF2α，8-异前列腺素F2α

Grape Seed Extract Given Three Hours After Injury Suppresses Lipid Peroxidation and Reduces Hypoxic-Ischemic Brain Injury in Neonatal Rats

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