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外文期刊>The biochemical journal
>Analogues of ribose 5-phosphate and 5-phosphoribosyl pyrophosphate. The preparation and properties of ribose 5-phosphorothioate and 5-phosphoribosyl 1-methylenediphosphonate
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Analogues of ribose 5-phosphate and 5-phosphoribosyl pyrophosphate. The preparation and properties of ribose 5-phosphorothioate and 5-phosphoribosyl 1-methylenediphosphonate
p1. 5-Phosphoribosyl 1-methylenediphosphonate was isolated after reaction of ribose 5-phosphate and iO/i-adenylyl methylenediphosphonate with 5-phosphoribosyl pyrophosphate synthetase from Ehrlich ascites-tumour cells. 2. The analogue reacted with adenine phosphoribosyltransferase, hypoxanthine phosphoribosyltransferase and nicotinamide phosphoribosyltransferase [iKsubm/sub/i (analogue)/iKsubm/sub/i (5-phosphoribosyl pyrophosphate) 0·17, 0·19 and 6·3 respectively; iV/isubmax./sub (analogue)/iV/isubmax./sub (5-phosphoribosyl pyrophosphate) 0·011, 0·26 and 1·1 respectively]. 3. The analogue was not a substrate for 5-phosphoribosyl pyrophosphate amidotransferase or orotate phosphoribosyltransferase. 4. Ribose 5-phosphorothioate was synthesized by allowing ribose to react with thiophosphoryl chloride in triethyl phosphate. The analogue was a substrate for 5-phosphoribosyl pyrophosphate synthetase from Ehrlich ascites-tumour cells. When this reaction was coupled to either adenine phosphoribosyltransferase or hypoxanthine phosphoribosyltransferase, adenosine 5′-phosphorothioate or inosine 5′-phosphorothioate was formed respectively./p
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机译：> 1。从Ehrlich腹水肿瘤细胞中分离出5-磷酸核糖5-磷酸核糖和亚甲基二膦酸O-腺苷酯与5-磷酸核糖基焦磷酸合成酶。 2.该类似物与腺嘌呤磷酸核糖基转移酶，次黄嘌呤磷酸核糖基转移酶和烟酰胺磷酸核糖基转移酶[ K m （类似物）/ K m （5-磷酸核糖基焦磷酸酯）分别为0·17、0·19和6·3; V max。（模拟）/ V max。（5-磷酸核糖基焦磷酸酯）0·011，0· 26和1·1]。 3.该类似物不是5-磷酸核糖焦磷酸酰胺基转移酶或乳清酸酯磷酸核糖基转移酶的底物。 4.通过使核糖与硫代磷酰氯在磷酸三乙酯中反应来合成核糖5-硫代磷酸酯。该类似物是来自Ehrlich腹水-肿瘤细胞的5-磷酸核糖基焦磷酸合成酶的底物。当该反应与腺嘌呤磷酸核糖基转移酶或次黄嘌呤磷酸核糖基转移酶偶合时，分别形成腺苷5'-硫代磷酸酯或肌苷5'-硫代磷酸酯。
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