pAn assay method was developed for the determination of high-affinity oestradiol receptors in uterine supernatant preparations. When only high-affinity sites are present in such preparations, or when they predominate, the analysis of the equilibrium between oestradiol and receptor sites based on the Scatchard (1949) plot may be used to determine the dissociation constant of the equilibrium and the molar concentration of the high-affinity sites. When both high-affinity and low-affinity sites are present the Scatchard plot is no longer linear and cannot be used directly to determine high-affinity sites. Determination of the reverse velocity constants of the reaction between high-affinity (ik/isub?1/sub) and low-affinity (ik/isub?2/sub) receptor sites and [sup3/supH]oestradiol has shown that these constants differ by at least one order of magnitude. Advantage has been taken of this difference to introduce an additional step into the assay procedure that eliminates oestradiol bound to low-affinity sites and permits the determination of high-affinity sites in different species and under a variety of physiological conditions./p
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机译：开发了一种测定子宫上清液制剂中高亲和性雌二醇受体的测定方法。当此类制备物中仅存在高亲和力位点或占主导地位时，可根据Scatchard（1949）图对雌二醇和受体位点之间的平衡进行分析，以确定平衡的解离常数和摩尔浓度高亲和力网站。当同时存在高亲和力和低亲和力位点时，Scatchard图不再是线性的，不能直接用于确定高亲和力的位点。确定高亲和力（ k ？1 ）和低亲和力（ k ？2）之间反应的反向速度常数）受体位点和[ 3 H]雌二醇已表明这些常数至少相差一个数量级。利用这种差异的优势，将测定步骤引入了额外的步骤，消除了与低亲和力位点结合的雌二醇，并允许在不同物种和各种生理条件下确定高亲和力位点。
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