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外文期刊>The biochemical journal
>Characterization of the β-lactamase specified by the resistance factor R-1818 in Escherichia coli K12 and other Gram-negative bacteria
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Characterization of the β-lactamase specified by the resistance factor R-1818 in Escherichia coli K12 and other Gram-negative bacteria
p1. The amino acid composition of the β-lactamase from iE. coli/i (R-1818) was determined. 2. The R-1818 β-lactamase is inhibited by formaldehyde, hydroxylamine, sodium azide, iodoacetamide, iodine and sodium chloride. 3. The iKsubm/sub/i values for benzylpenicillin, ampicillin and oxacillin have been determined by using the R-factor enzyme from different host species. The same values were obtained, irrespective of the host bacterium. 4. The molecular weight of the enzyme was found to be 44600, and was the same for all host species. 5. The relationship of R-1818 and R-GN238 β-lactamases is discussed./p
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机译：> 1。来自E的β-内酰胺酶的氨基酸组成。确定了大肠杆菌（R-1818）。 2. R-1818β-内酰胺酶被甲醛，羟胺，叠氮化钠，碘乙酰胺，碘和氯化钠抑制。 3.苄青霉素，氨苄青霉素和奥沙西林的 K m 值已使用来自不同宿主物种的R因子酶确定。无论宿主细菌是什么，都获得相同的值。 4.发现该酶的分子量为44600，并且对于所有宿主物种都是相同的。 5.探讨了R-1818与R-GN238β-内酰胺酶的关系。
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