pThe amino acid sequences of sesame (iSesamum indicum/i L.) and castor (iRicinus communis/i L.) cytochrome ic/i were determined by using 1.5μmol of protein from each species. Both molecules consist of a single chain of 111 amino acid residues and are homologous with other mitochondrial cytochrome ic/i molecules. Both have an iN/i-acetylated ‘tail’ of eight amino acids and two ∈-iN/i-trimethyl-lysine residues, as also reported for wheat germ (Delange, Glazer & Smith, 1969) and mung-bean cytochrome ic/i (Thompson, Laycock, Ramshaw & Boulter, 1970). Two different preparations of castor cytochrome ic/i differed by one residue. This was glutamic acid for glutamine in position 100. The results for sesame and castor cytochrome ic/i led to a re-examination and subsequent correction to the iN/i-terminal region of the mung-bean cytochrome ic/i sequence, as given by Thompson iet al./i (1970)./p
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机译:>芝麻( Sesamum indicum i> L.)和蓖麻( Ricinus communis i> L.)细胞色素 c i>的氨基酸序列通过每个物种1.5μmol的蛋白质。两种分子均由一条111个氨基酸残基的单链组成,并且与其他线粒体细胞色素 c i>分子同源。两者都具有由八个氨基酸组成的 N i>-乙酰化的“尾巴”和两个ε- N i>-三甲基赖氨酸残基,这在小麦胚芽中也有报道(Delange,Glazer&史密斯(1969)和绿豆细胞色素 c i>(汤普森,莱考克,拉姆肖和鲍尔特(1970))。蓖麻细胞色素 c i>的两种不同制备方法相差一个残基。这是位置100的谷氨酰胺的谷氨酸。芝麻和蓖麻细胞色素 c i>的结果导致重新检查并随后校正了绿豆的 N i>端区域豆细胞色素 c i>序列,由汤普森等人 i>(1970)给出。 p>
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