pUltraturrax- and Nagarse-released populations of mitochondria were characterized with respect to their Ca2+-uptake activities (i) by means of the indirect polarographic technique and (ii) directly by the 45Ca Ruthenium Red-quench method of Reed & Bygrave [(1974) Biochem. J. 140, 143-155]. The denervated-muscle subsarcolemmal and intermyofibrillar mitochondrial fractions displayed markedly decreased rates and capacities for Ca2+ uptake compared with their respective controls. The implications of these findings with respect to the process of cell necrosis are discussed./p
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机译:线粒体的超turrarax和Nagarse释放群体的钙离子吸收活性(i)通过间接极谱技术和(ii)通过Reed& Co.的45Ca钌红猝灭法直接表征。 Bygrave [(1974)Biochem。 J. 140,143-155]。与各自的对照相比,去神经的肌膜下肌层和肌原纤维间线粒体级分显示出降低的钙吸收速率和能力。讨论了这些发现对细胞坏死过程的意义。 p>
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