p1. The finding that the plant is the genetic determinant of leghaemoglobin production in legume nodules was further tested by inoculating snake beans with two strains of iRhizobium/i selected to give large genetic differences. Carbohydrate requirement patterns, immunological techniques and DNA base ratio determinations were used to demonstrate genetic differences between the two rhizobial strains. 2. Partially purified preparations of the haemoglobins from the nodules produced by the two strains showed no differences when examined by electrophoresis, isoelectric focusing or ion-exchange chromatography. 3. Two different leghaemoglobins from each type of nodule were separated by chromatography on DEAE-cellulose. One of these was isolated in the Fesup3+/sup form and accounted for two-thirds of the total leghaemoglobin. When it was examined in the analytical ultracentrifuge and by amino acid analysis, this major component did not vary with the inoculant rhizobial strain. The molecule had an is/isub20,w/sub of 1.88S, a diffusion coefficient of 10.7×10sup-7/supcmsup2/sup· ssup-1/sup and a mol. wt. of 16700. 4. These results strongly support the hypothesis that the mRNA for leghaemoglobin is transcribed from plant DNA./p
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机译：> 1。该植物是豆科植物结节中高铁血红蛋白产生的遗传决定因素，这一发现通过用两种选择的具有较大遗传差异的 Rhizobium 菌株接种蛇豆来进一步测试。碳水化合物的需求模式，免疫学技术和DNA碱基比率测定被用来证明这两种根瘤菌菌株之间的遗传差异。 2.当通过电泳，等电聚焦或离子交换色谱法检查时，由两种菌株产生的结节部分纯化的血红蛋白制剂没有显示出差异。 3.在DEAE-纤维素上通过色谱分离来自每种结节的两种不同的血红蛋白。其中之一以Fe 3 + 的形式分离，占总血红蛋白的三分之二。在分析型超速离心机中通过氨基酸分析对其进行检查时，该主要成分不会随接种的根瘤菌菌株而变化。该分子的 s 20，w 为1.88S，扩散系数为10.7×10 -7 cm 2 ·s -1 和一摩尔。重量大约是16700。4.这些结果强有力地支持了从植物DNA转录来血红蛋白的mRNA的假说。
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