Triticum aestivum L. endoxylanase inhibitor (TAXI) consists of two inhibitors, TAXI I and TAXI II, with different specificities

Hans GOESAERT; Jan A. DELCOUR; Jozef VAN DAMME; Kurt GEBRUERS; Paul PROOST; Winok DEBYSER

摘要

pThe iTriticum aestivum/i L. endoxylanase inhibitor (TAXI) discovered by Debyser and Delcour [(1997) Eur. Pat. filed April 1997, published as WO 98/49278] and Debyser, Derdelinckx and Delcour [(1997) J. Am. Soc. Brew. Chem. b55/b, 153?156] seems to be a mixture of two different endoxylanase inhibitors, called TAXI I and TAXI II. By using iAspergillus niger/i as well as iBacillus subtilis/i endoxylanases for assaying inhibition activity, both inhibitors could be purified to homogeneity from wheat (iTriticum aestivum/i L., var. Soissons). TAXI I and TAXI II have similar molecular structures. They both have a molecular mass of approx. 40.0kDa, are not glycosylated and occur in two molecular forms, i.e. a non-proteolytically processed one and a proteolytically processed one. However, the pI of TAXI II (at least 9.3) is higher than that of TAXI I (8.8). TAXI I and TAXI II clearly show different inhibition activities towards different endoxylanases. The N-terminal amino acid sequences of both inhibitors show a high degree of identity, which might indicate that there is an evolutionary relationship between them./p

机译：> Debyser和Delcour发现的普通小麦内切木聚糖酶（TAXI）[（1997）Eur。拍。 1997年4月提交，公开为WO 98/49278]和Debyser，Derdelinckx和Delcour [（1997）J. Am。 Soc。酿造。化学 55 ，153？156]似乎是两种不同的木聚糖内切酶抑制剂TAXI I和TAXI II的混合物。通过使用黑曲霉（Aspergillus niger）和枯草芽孢杆菌（Bacillus subtilis）内切木聚糖酶来测定抑制活性，可以将两种抑制剂从小麦（ Triticum aestivum L. ，var。Soissons）。 TAXI I和TAXI II具有相似的分子结构。它们两者的分子质量均为约。 40.0kDa未被糖基化并且以两种分子形式出现，即非蛋白水解加工的一种和蛋白水解加工的一种。但是，TAXI II的pI（至少9.3）高于TAXI I的pI（8.8）。 TAXI I和TAXI II清楚地显示出对不同内切木聚糖酶的不同抑制活性。两种抑制剂的N端氨基酸序列具有高度的同一性，这可能表明它们之间存在进化关系。

Triticum aestivum L. endoxylanase inhibitor (TAXI) consists of two inhibitors, TAXI I and TAXI II, with different specificities

摘要

著录项

相关主题

期刊订阅