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外文期刊>The biochemical journal
>Cadmium regulates copper homoeostasis by inhibiting the activity of Mac1, a transcriptional activator of the copper regulon, in Saccharomyces cerevisiae
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Cadmium regulates copper homoeostasis by inhibiting the activity of Mac1, a transcriptional activator of the copper regulon, in Saccharomyces cerevisiae
pCadmium is a toxic metal and the mechanism of its toxicity has been studied in various model systems from bacteria to mammals. We employed iSaccharomyces cerevisiae/i as a model system to study cadmium toxicity at the molecular level because it has been used to identify the molecular mechanisms of toxicity found in higher organisms. cDNA microarray and Northern blot analyses revealed that cadmium salts inhibited the expression of genes related to copper metabolism. Western blotting, Northern blotting and chromatin immunoprecipitation experiments indicated that iCTR1/i expression was inhibited at the transcriptional level through direct inhibition of the Mac1 transcriptional activator. The decreased expression of iCTR1/i results in cellular copper deficiency and inhibition of Fet3 activity, which eventually impairs iron uptake. In this way, cadmium exhibits a negative effect on both iron and copper homoeostasis./p
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