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首页> 外文期刊>The Journal of Experomental Medicine >Alloreactive cytolytic T cell clones with dual recognition of HLA-B27 and HLA-DR2 antigens. Selective involvement of CD8 in their class I--directed cytotoxicity.
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Alloreactive cytolytic T cell clones with dual recognition of HLA-B27 and HLA-DR2 antigens. Selective involvement of CD8 in their class I--directed cytotoxicity.

机译：具有HLA-B27和HLA-DR2抗原双重识别的同种反应性溶细胞性T细胞克隆。 CD8选择性参与其I类-细胞毒性。

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HLA-B27- responder cells were stimulated in vitro with HLA-B27.1+ lymphoblastoid cell lines, and alloreactive CTL clones were obtained by limiting dilution. Three of these clones specifically lysed B27.1+ targets. In addition, they also lysed homozygous DR2 targets with various degrees of efficiency, depending on the Dw specificity of the target cell. All three clones possessed a homogeneous CD3+,CD8+,CD4- phenotype and were also homogeneous upon subcloning. Cold-target inhibition analyses showed mutual inhibition of B27.1 target lysis by DR2 targets and vice versa. Lysis of B27.1 targets was selectively inhibited by anti-class I mAbs. In contrast, lysis of DR2 targets was inhibited only by anti-class II and anti-DR monomorphic antibodies, but not by anti-class I, anti-DQw1, or anti-DP antibodies. The results indicate that these clones display dual recognition for HLA-B27.1 and for HLA-DR2 and suggest that HLA-B27.1 may share at least one epitope that is closely related to some stimulatory Dw determinants present on the HLA-DR2 antigens. Lysis of both B27+ and DR+ targets was inhibited by an anti-CD3 mAb. In contrast, an anti-CD8 antibody selectively inhibited the B27- but not the DR2-directed killing by these clones. The data support a stabilizing role of CD8 through its binding to the same class I (but not class II) molecule on the target cell bound by the T cell antigen receptor.
机译：用HLA-B27.1 +淋巴母细胞样细胞系体外刺激HLA-B27-应答细胞，并通过有限稀释获得同种反应性CTL克隆。这些克隆中的三个特异性裂解了B27.1 +靶标。此外，它们还根据靶细胞的Dw特异性以不同效率裂解纯合DR2靶。这三个克隆均具有均一的CD3 +，CD8 +，CD4-表型，并且在亚克隆时也均一。冷靶标抑制分析表明DR2靶标对B27.1靶标的裂解具有相互抑制作用，反之亦然。抗I类mAb选择性抑制B27.1靶标的裂解。相反，DR2靶标的溶解仅被抗II类和抗DR单态抗体抑制，而不受抗I类，抗DQw1或抗DP抗体抑制。结果表明，这些克隆显示出对HLA-B27.1和HLA-DR2的双重识别，并表明HLA-B27.1可能共享至少一个与HLA-DR2抗原上存在的一些刺激性Dw决定簇密切相关的表位。 B27 +和DR +靶标的溶解均被抗CD3 mAb抑制。相反，抗CD8抗体选择性抑制这些克隆对B27的杀伤，但对DR2的杀伤没有抑制作用。数据通过将CD8结合到与T细胞抗原受体结合的靶细胞上的同一I类（而非II类）分子来支持CD8的稳定作用。

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