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首页> 外文期刊>World Journal of Gastroenterology >Influence of CXCR4/SDF-1 axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells
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Influence of CXCR4/SDF-1 axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells

机译：CXCR4 / SDF-1轴对HT29结肠癌细胞E-cadherin /β-catenin复合物表达的影响

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AIM: To study the influence of CXCR4/stromal cell-derived factor-1 (SDF-1) axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells and its underlying mechanisms. METHODS: Effect of SDF-1 on E-cadherin/β-catenin expression was detected by immunocytochemistry. E-cadherin and β-catenin mRNA expression levels were measured by reverse transcriptase-polymerase chain reaction. SDF-1-induced phosphorylation of phosphatidylinositol 3-kinase (PI3K)/AKT and β-catenin was detected by Western blotting. RESULTS: The E-cadherin and β-catenin mRNA expression levels in HT29 cells were lower 48 h after incubated with SDF-1 at the concentrations of 20 and 40 ng/mL (P CONCLUSION: SDF-1 down-regulates the E-cadherin/β-catenin complex expression in HT29 cells by decreasing mRNA synthesis and increasing β-catenin phosphorylation.
机译：目的：研究CXCR4 /基质细胞源因子-1（SDF-1）轴对HT29结肠癌细胞E-cadherin /β-catenin复合物表达的影响及其潜在机制。方法：采用免疫细胞化学法检测SDF-1对E-cadherin /β-catenin表达的影响。通过逆转录酶-聚合酶链反应测量E-钙粘蛋白和β-连环蛋白的mRNA表达水平。通过Western印迹检测SDF-1诱导的磷脂酰肌醇3-激酶（PI3K）/ AKT和β-连环蛋白的磷酸化。结果：与浓度为20和40 ng / mL的SDF-1孵育48小时后，HT29细胞中的E-钙粘蛋白和β-cateninmRNA表达水平降低（P结论：SDF-1下调了E-钙粘蛋白/β-catenin复合物在HT29细胞中的表达通过减少mRNA合成和增加β-catenin磷酸化来实现。

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