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Evaluation of Twenty-Three Blood Culture Media

机译：23种血液培养基的评价

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Several investigators have evaluated clinically a variety of commercially available blood culture media. No agreement has been reached as to which of these media is optimal for detection of bacteremia. The purpose of this study was to determine the rate of recovery of microorganisms from various blood culture media. A total of 23 blood culture media were inoculated with 7 to 15 microorganisms per bottle in the presence or absence of an erythrocyte-serum mixture. The results demonstrated that blood culture media differed in their ability to support the growth of microorganisms. At 4 days after inoculation, only 10 of the 23 blood culture media supported the growth of 91% (10 of the 11) or more of the test microorganisms. The recovery rate of microorganisms depended not only upon the type of medium but also upon the manufacturer of the type of blood culture medium. The addition of an erythrocyte-serum mixture to the blood culture media did not influence the difference in the recovery rate of microorganisms among media and the same type of medium prepared by different manufacturers. The majority (15 of the 23) of the blood culture media supplemented with the erythrocyte-serum mixture failed to support the growth of 91% or more of the test microorganisms at 4 days after inoculation. These results have demonstrated that blood culture media need to be improved. Better quality control measures should also be implemented to evaluate commercial blood culture media.
机译：几位研究者已经在临床上评估了各种可商购的血液培养基。关于哪种培养基最适合检测菌血症尚未达成共识。这项研究的目的是确定从各种血液培养基中回收微生物的速率。在存在或不存在红细胞-血清混合物的情况下，每瓶总共向23种血液培养基接种7至15种微生物。结果表明，血液培养基支持微生物生长的能力不同。接种后第4天，在23种血液培养基中，只有10种支持91％（11种中的10种）或更多测试微生物的生长。微生物的回收率不仅取决于培养基的类型，而且取决于血液培养基的类型的制造商。向血液培养基中添加红细胞-血清混合物不会影响培养基与不同制造商制备的相同类型培养基之间微生物回收率的差异。补充有红细胞-血清混合物的大多数血液培养基（23种中的15种）在接种后4天不能支持91％或更多的测试微生物的生长。这些结果表明，血液培养基需要改进。还应实施更好的质量控制措施，以评估商业血液培养基。

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